HSF1 anticorps

N° du produit ABIN453930

Détail du produit anti-HSF1 anticorps

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin, Singe, Cobaye, Hamster

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSF1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Specificité: This antibody detects an ~85 kDa protein in unstressed cell lysates, and an ~95 kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat, Bovine, Guinea pig, Hamster, Monkey, Rabbit

Attributs du produit: Synonyms: HSTF1, HSF-1, Heat shock transcription factor 1

Purification: Purified

Immunogène: Purified recombinant HSF1 protein

Clone: 10H8

Isotype: IgG1

Information d'application

Indications d'application: Western blot (see ref.1). Immunoprecipitation. Immunocytochemistry (see ref.1). ELISA (see ref.1). Gel Mobility Shift Assay (see ref.1).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % sodium azide in 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur HSF1

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Autre désignation: Heat Shock Factor 1 / HSF1 (HSF1 Produits)

Synonymes: anticorps hsf1, anticorps Hsf, anticorps xhsf1, anticorps AA960185, anticorps HSTF1, anticorps HSF1, anticorps HSF, anticorps HSF1c, anticorps hsf1a, anticorps hsf1b, anticorps zHSF1a, anticorps zHSF1b, anticorps hstf1, anticorps heat shock factor, anticorps Heat Shock Factor, anticorps heat shock factor 1, anticorps heat shock transcription factor 1, anticorps heat shock transcription factor 1 S homeolog, anticorps hsf1, anticorps hsf-1, anticorps Hsf1, anticorps HSF1, anticorps hsf1.S

Sujet: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

ID gène: 3297

UniProt: Q00613