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ELANE anticorps (AA 27-265)

ELANE Reactivité: Souris, Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN4886568
  • Antigène Voir toutes ELANE Anticorps
    ELANE (Elastase, Neutrophil Expressed (ELANE))
    Épitope
    • 16
    • 11
    • 7
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 27-265
    Reactivité
    • 88
    • 24
    • 23
    • 4
    • 1
    Souris, Rat
    Hôte
    • 67
    • 19
    • 4
    • 1
    Lapin
    Clonalité
    • 62
    • 29
    Polyclonal
    Conjugué
    • 46
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ELANE est non-conjugé
    Application
    • 54
    • 39
    • 28
    • 17
    • 13
    • 10
    • 9
    • 9
    • 6
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Fonction
    Rabbit IgG polyclonal antibody for Neutrophil elastase(ELANE) detection. Tested with WB, IHC-P in Mouse,Rat.
    Réactivité croisée (Details)
    No cross reactivity with other proteins.
    Attributs du produit
    Rabbit IgG polyclonal antibody for Neutrophil elastase(ELANE) detection. Tested with WB, IHC-P in Mouse,Rat.
    Gene Name: elastase, neutrophil expressed
    Protein Name: Neutrophil elastase
    Purification
    Immunogen affinity purified.
    Immunogène
    E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265). Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2.
    Isotype
    IgG
  • Indications d'application
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Commentaires

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Antigène
    ELANE (Elastase, Neutrophil Expressed (ELANE))
    Autre désignation
    ELANE (ELANE Produits)
    Synonymes
    anticorps ELA2, anticorps GE, anticorps HLE, anticorps HNE, anticorps NE, anticorps PMN-E, anticorps SCN1, anticorps Ela2, anticorps F430011M15Rik, anticorps ela2, anticorps ELANE, anticorps elastase, neutrophil expressed, anticorps proteinase 3, anticorps ELANE, anticorps Elane, anticorps prtn3
    Sujet
    Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

    Synonyms: ELA2 | ELANE | Elastase 2 | Elastase-2 | HLE | HNE | Leukocyte elastase | Medullasin | Neutrophil elastase | PMN E | PMN elastase | SCN1 | P08246
    ID gène
    50701
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