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PUMA anticorps (C-Term)

BBC3 Reactivité: Humain, Souris WB, IF, EIA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN500557
  • Antigène Voir toutes PUMA (BBC3) Anticorps
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Épitope
    • 20
    • 18
    • 16
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 113
    • 40
    • 38
    • 16
    Humain, Souris
    Hôte
    • 109
    • 7
    • 1
    Lapin
    Clonalité
    • 98
    • 19
    Polyclonal
    Conjugué
    • 52
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PUMA est non-conjugé
    Application
    • 98
    • 40
    • 39
    • 22
    • 22
    • 17
    • 16
    • 14
    • 13
    • 9
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Specificité
    This antibody detects PUMA at C-term.
    Réactivité croisée (Details)
    Species reactivity (tested):Human, Mouse.
    Purification
    Peptide Affinity Chromatography
    Immunogène
    Synthetic peptide corresponding to 14 amino acids near the carboxy terminus of Human PUMA alpha. .Remarks: The sequence is identical between alpha and beta forms of the PUMA proteins.
    Isotype
    IgG
    Top Product
    Discover our top product BBC3 Anticorps primaire
  • Indications d'application
    ELISA. Western blot: 1 - 2 μg/mL. K562 or 3T3 cell lysate can be used as a positive control and aband at approximately 23 kDa can be detected. A lower band at approximately 16 kDa wasdetected in MOLT4 and U937 cells, which may represent the PUMA-b form. Immunocytochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS, 0.02 % Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Autre désignation
    PUMA (BBC3 Produits)
    Synonymes
    anticorps BBC3, anticorps Puma, anticorps JFY-1, anticorps JFY1, anticorps PUMA, anticorps PUMA/JFY1, anticorps BCL2 binding component 3, anticorps Bcl-2 binding component 3, anticorps BBC3, anticorps Bbc3, anticorps bbc3
    Sujet
    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
    Poids moléculaire
    23 kDa
    ID gène
    27113
    NCBI Accession
    NP_055232
    UniProt
    Q9BXH1
    Pathways
    Signalisation p53, Positive Regulation of Endopeptidase Activity
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