E2F1 anticorps (pSer364)
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- Antigène Voir toutes E2F1 Anticorps
- E2F1 (E2F Transcription Factor 1 (E2F1))
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Épitope
- AA 360-369, pSer364
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Reactivité
- Humain, Chimpanzé
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp E2F1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificité
- This antibody is directed against the phosphorylated form of E2F-1 at the pS364 residue. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites.
- Réactivité croisée (Details)
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Species reactivity (expected):Chimpanzee 100 % .
Species reactivity (tested):Human. - Purification
- The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
- Immunogène
- Synthetic peptide corresponding to amino acids 360-369 of Human E2F-1
- Isotype
- IgG
- Top Product
- Discover our top product E2F1 Anticorps primaire
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- Indications d'application
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ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 250 - 1: 2,000. Immunohistochemistry on paraffin sections: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.80 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- Antigène
- E2F1 (E2F Transcription Factor 1 (E2F1))
- Autre désignation
- E2F1 (E2F1 Produits)
- Synonymes
- anticorps CG6376, anticorps DRTF1/E2F, anticorps DmE2F-1, anticorps Dmel\\CG6376, anticorps Dp, anticorps E(Sev-CycE)3A, anticorps E(var)3-93E, anticorps E(var)3-95E, anticorps E(var)93E, anticorps E2F, anticorps E2F-1, anticorps E2F1, anticorps E2f1, anticorps Evar(3)164, anticorps dE2F, anticorps dE2F1, anticorps dE2f, anticorps dE2f1, anticorps de2f1, anticorps def21, anticorps drosE2F1, anticorps e2f, anticorps e2f1, anticorps l(3)07172, anticorps l(3)j3B1, anticorps l(3)j3C2, anticorps l(3)rM729, anticorps RBAP1, anticorps RBBP3, anticorps RBP3, anticorps mKIAA4009, anticorps xE2F, anticorps ATE2FB, anticorps E2F TRANSCRIPTION FACTOR-1 E2F1, anticorps E2F transcription factor 1, anticorps E2FB, anticorps E2FB TRANSCRIPTION FACTOR, anticorps E2F transcription factor 1, anticorps E2F transcription factor 1 L homeolog, anticorps E2f1, anticorps E2F1, anticorps e2f1.L
- Sujet
- E2F-1 (also known as tran-scription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains con-served through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain that determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein as well as E2F2 and E2F3 have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner and mediates both cell proliferation and p53-dependent/independent apoptosis. Increased nuclear expression of this protein has been reported in a variety of cancers.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
- ID gène
- 1869
- NCBI Accession
- NP_005216
- UniProt
- Q01094
- Pathways
- Signalisation p53, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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