Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CRISPR-Cas9 anticorps

Reactivité: Streptococcus pyogenes WB Hôte: Souris Monoclonal 11E7- unconjugated
N° du produit ABIN5563966
  • Antigène
    CRISPR-Cas9
    Reactivité
    • 20
    • 2
    • 1
    • 1
    • 1
    • 1
    Streptococcus pyogenes
    Hôte
    • 16
    • 10
    Souris
    Clonalité
    • 13
    • 13
    Monoclonal
    Conjugué
    • 18
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Inconjugué
    Application
    • 24
    • 10
    • 8
    • 7
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Purification
    Affinity Purified
    Clone
    11E7-
    Isotype
    IgG2a
  • Indications d'application
    Validated Applications: WB: 0.5 - 2 μg/mL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
  • Antigène
    CRISPR-Cas9
    Autre désignation
    Cas9
    Sujet
    SaCas9 is a nuclease from Staphylococcus aureus that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
    Poids moléculaire
    130 kDa
Vous êtes ici:
Support technique