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His Tag anticorps

Détails pour le produit réf. ABIN559683, Fournisseur: Connectez-vous pour afficher Nouveau
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Antigène
Synonymes AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI
Reactivité
Tag
(486), (23), (7), (3)
Hôte
Souris
(276), (181), (32), (26), (3)
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
(51), (39), (37), (15), (12), (9), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Dot Blot (DB), Immunostaining (ISt), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(436), (279), (168), (76), (65), (35), (33), (13), (13), (11), (8), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 références disponible
Fournisseur Connectez-vous pour afficher Nouveau
Numéro du produit fournisseur Connectez-vous pour afficher Nouveau
Quantité 100 μg
Destination France ( )
Disponibilité Envoi sous 5 à 7 jours ouvrables
Immunogène HHHHHH (6x His) synthetic peptide conjugated to KLH
Séquence HHHHHH
Clone HIS-H8
Isotype IgG2b
Specificité Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Sensibilité WB (with ECL): 1-2ng of His-tagged fusion proteins
Purification Protein A affinity chromatography from mouse ascites fluid
Classe de substances Tag
Domaine de recherche Tags/Labels
Pathways
Indications d'application WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Restrictions For Research Use only
Concentration 1mg/mL
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Supplier Images
 image for anti-His Tag anticorps (ABIN559683) LEFT: untransfected control, RIGHT: anti-His (in red) on His-tagged fusion proteins i...
 image for anti-His Tag anticorps (ABIN559683) Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, pro...
 image for anti-His Tag anticorps (ABIN559683) Standard ladder containing five different His-tagged proteins, untransfected control ...
Produit citée dans: Chi, Bo, Wu et al.: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." dans: PLoS ONE, Vol. 7, Issue 4, pp. e35862, 2012 (PubMed).

Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." dans: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Green, Reed: "Mitochondria and apoptosis." dans: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri et al.: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." dans: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Reference: Background Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." dans: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." dans: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." dans: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

N° du produit ABIN559683
160,89 €
Plus frais de livraison 40,00 € et TVA

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