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STRO-1 anticorps

Reactivité: Humain, Lapin, Rat FACS, ICC Hôte: Souris Monoclonal STRO-1 unconjugated
N° du produit ABIN638439
  • Antigène
    STRO-1
    Reactivité
    Humain, Lapin, Rat
    Hôte
    • 7
    Souris
    Clonalité
    • 7
    Monoclonal
    Conjugué
    • 3
    • 3
    • 1
    Inconjugué
    Application
    • 7
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificité
    The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
    Réactivité croisée (Details)
    Human
    Purification
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    Human CD34 positive bone marrow cells
    Clone
    STRO-1
    Isotype
    IgM lambda
  • Indications d'application
    Flow cytometry: Recommended dilution: 1-5 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Kaneko, Akita, Shimauchi, Sasano: "Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy." dans: Journal of electron microscopy, Vol. 58, Issue 6, pp. 363-73, (2009) (PubMed).

    Seo, Miura, Sonoyama, Coppe, Stanyon, Shi: "Recovery of stem cells from cryopreserved periodontal ligament." dans: Journal of dental research, Vol. 84, Issue 10, pp. 907-12, (2005) (PubMed).

    Bensidhoum, Chapel, Francois, Demarquay, Mazurier, Fouillard, Bouchet, Bertho, Gourmelon, Aigueperse, Charbord, Gorin, Thierry, Lopez: "Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment." dans: Blood, Vol. 103, Issue 9, pp. 3313-9, (2004) (PubMed).

    Gronthos, Zannettino, Hay, Shi, Graves, Kortesidis, Simmons: "Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow." dans: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1827-35, (2003) (PubMed).

    Stenderup, Justesen, Eriksen, Rattan, Kassem: "Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis." dans: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 16, Issue 6, pp. 1120-9, (2001) (PubMed).

    Oyajobi, Lomri, Hott, Marie: "Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody." dans: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 14, Issue 3, pp. 351-61, (1999) (PubMed).

    Simmons, Torok-Storb: "Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1." dans: Blood, Vol. 78, Issue 1, pp. 55-62, (1991) (PubMed).

  • Antigène
    STRO-1
    Sujet
    STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10 % of mononuclear cells, greater than 95 % of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.,STRO1
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