ATP1A2 anticorps (AA 451-479)

N° du produit ABIN652011

Détail du produit anti-ATP1A2 anticorps

Antigène: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Épitope: AA 451-479

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP1A2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Homologie: B, Pig, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This ATP1A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human ATP1A2.

Clone: RB19033

Isotype: Ig Fraction

Information d'application

Indications d'application: IF: 1:10~50. WB: 1:2000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Independent Validation (IF)

Validation #100013 (Immunofluorescence)

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100013

Date: 11.05.2016

Antigène: ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2)

Numéro du lot: SH09708BF

Application validée: Immunofluorescence

Contrôle positif: Wild-type mouse adult brain cryo-sections

Contrôle négative: Rabbit IgG

Conclusion: The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.

Validation Images

IF validation of ATP1A2 antibody ABIN652011 on mouse brain sections. A. ABIN652011 diluted 1:10 (red), DAPI counterstaining (blue). B. Dilution factor 1:10. C. Dilution factor 1:50. D. Dilution factor 1:200.

Protocole

Anticorps primaire: ABIN652011

Anticorps secondaire: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• • Dry slides at 50-55°C for 15-30min (or RT for 30min).
  • Wash with PBS 5min X 3 (shake gently).
  • Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
  • Incubate with ABIN652011 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
  • Wash with PBS 5min X 3.
  • Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
  • Wash with PBS 5min X 3.
  • Add three drops of water-based Immu-mount.
  • Cover with 24 X 60 cover glass.
• Imaging:
• • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Notes: ABIN652011 worked at 1:10, did not work well in 1:50-1:200.

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Références pour anticorps anti-ATP1A2 (ABIN652011)

Du, Kiyoshi, Wang, Wang, Ma, Alford, Zhong, Wan, Chen, Lloyd, Bryan, Zhou: "Genetic Deletion of TREK-1 or TWIK-1/TREK-1 Potassium Channels does not Alter the Basic Electrophysiological Properties of Mature Hippocampal Astrocytes In Situ." dans: Frontiers in cellular neuroscience, Vol. 10, pp. 13, (2016) (PubMed).

Wang, Kiyoshi, Du, Ma, Alford, Chen, Zhou: "mGluR3 Activation Recruits Cytoplasmic TWIK-1 Channels to Membrane that Enhances Ammonium Uptake in Hippocampal Astrocytes." dans: Molecular neurobiology, (2015) (PubMed).

Détails sur ATP1A2

Antigène: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Autre désignation: ATP1A2 (ATP1A2 Produits)

Poids moléculaire: 112265

ID gène: 477

NCBI Accession: NP_000693

UniProt: P50993

Pathways: Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process