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MME anticorps (AA 501-600)

Name: MME anticorps (AA 501-600)
SKU: ABIN669666
Price: 414.62 EUR
Availability: InStock

MME Reactivité: Humain, Souris, Rat FACS, WB, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-CD10 Polyclonal Antibody (ABIN669666), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

Human Hela cells probed with CD10 Polyclonal Antibody, Alexa Fluor 647 conjugated -A647) (green) at 1:20 for 30 minutes compared to unstained cells (blue) and isotype control (orange).

FACS

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD10 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

A549 cells probed with CD10 Polyclonal Antibody, Unconjugated at 1:20 for 30 minutes followed by incubation with a conjugated secondary -PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

FACS

5 images

(1 reference)

N° du produit ABIN669666

Fiche de données

Antigène Voir toutes MME Anticorps

MME (Membrane Metallo-Endopeptidase (MME))
Épitope
15

14

10

8

8

7

7

7

7

6

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 501-600
Reactivité
213

57

49

3

2

2

2

2

2

1

1

1

1
Humain, Souris, Rat
Hôte
110

109

2
Lapin
Anticorps secondaires appropriés
Clonalité
116

104

2
Polyclonal
Conjugué
108

19

19

14

9

6

5

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp MME est non-conjugé
Application
132

100

80

68

38

29

21

20

12

12

10

8

5

5

5

3

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Réactivité croisée

Humain, Souris, Rat

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from human CD10

Isotype

IgG

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MME Reactivité: Humain FACS, WB, IHC, ELISA, ICC, Neut Hôte: Souris Monoclonal 6G12E12 unconjugated

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MME Reactivité: Humain WB, IHC, ELISA, StM, Coat Hôte: Souris Monoclonal MME-1892 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody
MME Reactivité: Humain, Souris, Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) antibody
MME Reactivité: Humain FACS, IP Hôte: Souris Monoclonal MEM-78 unconjugated

Indications d'application

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Chopra, Kraus, Schwinn, Ritz, Mattenheimer, Mottok, Rosenwald, Einsele, Beilhack: "Non-invasive bioluminescence imaging to monitor the immunological control of a plasmablastic lymphoma-like B cell neoplasia after hematopoietic cell transplantation." dans: PLoS ONE, Vol. 8, Issue 12, pp. e81320, (2013) (PubMed).
Antigène

MME (Membrane Metallo-Endopeptidase (MME))

Autre désignation

CD10 (MME Produits)

Synonymes

anticorps CALLA, anticorps CD10, anticorps NEP, anticorps SFE, anticorps 6030454K05Rik, anticorps C85356, anticorps Nep, anticorps neprilysin, anticorps membrane metalloendopeptidase, anticorps membrane metallo endopeptidase, anticorps membrane metallo-endopeptidase, anticorps MME, anticorps Mme

Classe de substances

Chemical

Sujet

Synonyms: NEP, SFE, CD1, CALLA, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Neutral endopeptidase, Skin fibroblast elastase, MME, EPN
Background: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 3 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

ID gène

4311

UniProt

P08473

Pathways

Signalisation RTK, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration