Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

DVL1 anticorps (AA 21-100)

DVL1 Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN670671
  • Antigène Voir toutes DVL1 Anticorps
    DVL1 (Dishevelled Segment Polarity Protein 1 (DVL1))
    Épitope
    • 15
    • 7
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 21-100
    Reactivité
    • 43
    • 23
    • 20
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 41
    • 2
    • 2
    Lapin
    Clonalité
    • 45
    Polyclonal
    Conjugué
    • 20
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp DVL1 est non-conjugé
    Application
    • 44
    • 18
    • 18
    • 13
    • 13
    • 12
    • 12
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Humain, Souris, Rat
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human DVL1
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029657 (Immunohistochemistry)
    'Independent Validation' signe
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029657
    Date
    03.04.2014
    Antigène
    Numéro du lot
    140106
    Application validée
    Immunohistochemistry
    Contrôle positif
    Human kidney tubules
    Contrôle négative
    Human colon stromal tissue
    Conclusion
    While faint, signal is detectable in positive control tissue and not in negative control tissue. Note that staining is expected in colon epithelial cells, and is not expected in kidney glomeruli.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antigen: Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1)
    • Catalog number: ABIN670671
    • Lot number: 140106
    Anticorps secondaire
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D07640BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 min
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
    • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
    • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 5. Slides rinsed with *reaction buffer* x3
    • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
    • 7. Slides rinsed with *reaction buffer*
    • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
    • 9. Slides rinsed with *reaction buffer* x3
    • 10. *iView Biotinylated IgG* applied and incubated for 8 min
    • 11. Slides rinsed with *reaction buffer*
    • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
    • 15. Slides rinsed with *reaction buffer*
    • 16. *iView DAB/H2O2* applied and incubated for 8 min
    • 17. Slides rinsed with *reaction buffer*
    • 18. *iView Copper* applied and incubated for 4 min
    • 19. Slides rinsed with *reaction buffer*
    • 20. Slides washed in Dawn Detergent/tap water
    • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
    • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
    • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Notes
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
    • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
    • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Hua, Xu, He, Jiang, Ye, Pan: "Wnt4/?-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1." dans: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8421-31, (2015) (PubMed).

  • Antigène
    DVL1 (Dishevelled Segment Polarity Protein 1 (DVL1))
    Autre désignation
    DVL1 (DVL1 Produits)
    Synonymes
    anticorps DVL, anticorps DVL1L1, anticorps DVL1P1, anticorps Dvl, anticorps mKIAA4029, anticorps dvl-1, anticorps DSH, anticorps DVL-1, anticorps dvl1, anticorps dvl2l, anticorps Xdsh, anticorps dsh1, anticorps dishevelled segment polarity protein 1, anticorps dishevelled, dsh homolog 1 (Drosophila), anticorps microRNA 6808, anticorps dishevelled segment polarity protein 1b, anticorps dishevelled segment polarity protein 1 L homeolog, anticorps DVL1, anticorps Dvl1, anticorps MIR6808, anticorps dvl1b, anticorps dvl1.L
    Sujet

    Synonyms: DVL, DVL1L1, DVL1P1, Segment polarity protein dishevelled homolog DVL-1, Dishevelled-1, DSH homolog 1, DVL1

    Background: Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).

    ID gène
    1855
    UniProt
    O14640
    Pathways
    Signalisation WNT, Synaptic Membrane, Skeletal Muscle Fiber Development
Vous êtes ici:
Support technique