DVL1 anticorps (Dishevelled, Dsh Homolog 1 (Drosophila)) (AA 20-70)

Details for Product anti-DVL1 Antibody No. ABIN670671, Fournisseur: Connectez-vous pour afficher
Antigène
  • Xdsh
  • dsh1
  • dvl-1
  • DVL
  • DVL1L1
  • DVL1P1
  • DSH
  • DVL-22
  • DVL1
  • DVL4
  • Dvl
  • mKIAA4029
  • DVL-1
  • dvl1
  • dvl2l
  • dishevelled segment polarity protein 1
  • dishevelled segment polarity protein 1 pseudogene 1
  • dishevelled, dsh homolog 1 (Drosophila)
  • dishevelled, dsh homolog 1b (Drosophila)
  • dvl1
  • Dvl1
  • DVL1
  • DVL1P1
  • dvl1b
Épitope
AA 20-70
14
14
10
10
9
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
61
44
28
17
9
5
4
3
3
2
2
2
1
1
1
Hôte
Lapin
61
23
3
Clonalité
Polyclonal
Conjugué
Cet anticorp DVL1 est non-conjugé
7
7
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
74
49
22
16
12
8
8
3
2
1
1
Options
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
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'Independent Validation' signe
Numéro du lot 140106
Application validée Immunohistochemistry
Contrôle positif Human kidney tubules
Contrôle négative Human colon stromal tissue
Conclusion While faint, signal is detectable in positive control tissue and not in negative control tissue. Note that staining is expected in colon epithelial cells, and is not expected in kidney glomeruli.
Anticorps primaire
Contrôle isotypique
Anticorps secondaire
Contrôles
  • Positive control: Human kidney tissue stained with antibody
  • Negative control: Human colon tissue stained with antibody
  • Isotype control: Human kidney tissue stained with isotype control
  • Secondary only control: Human kidney tissue stained with secondary antibody only
Protocole
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Notes
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 1: Human kidney tissue stained with anti-DVL1 (brown) and counterstained with ...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 2: Human colon tissue stained with anti-DVL1 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 3: Human kidney tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 4: Human kidney tissue stained with secondary only (brown) and counterstained ...
Immunogène KLH conjugated synthetic peptide derived from human DVL1
Isotype IgG
Purification Purified by Protein A.
Autre désignation DVL1 (DVL1 Antibody Extrait)
Sujet

Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).

Subcellular location: Cytoplasm, Cell membrane

Synonyms: DVL, DVL1L1, DVL1P1, Segment polarity protein dishevelled homolog DVL-1, Dishevelled-1, DSH homolog 1, DVL1

ID gène 1855
UniProt O14640
Pathways Signalisation WNT, Synaptic Membrane, Skeletal Muscle Fiber Development
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Supplier Images
SDS-PAGE (SDS) image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Lane 1: rat brain lysates Lane 2: rat lung lysates probed with Anti DVL1/Dishevelled ...
Immunohistochemistry (IHC) image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Images provided the Independent Validation Program (badge number 029657)Formalin-fixe...
Produit citée dans: Hua, Xu, He, Jiang, Ye, Pan: "Wnt4/?-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1." dans: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8421-31, 2015 (PubMed).

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