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WNT2B anticorps (AA 301-391)

WNT2B Reactivité: Souris, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN675728
  • Antigène Voir toutes WNT2B Anticorps
    WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))
    Épitope
    • 14
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 301-391
    Reactivité
    • 35
    • 32
    • 30
    • 8
    • 7
    • 7
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    Souris, Rat
    Hôte
    • 48
    • 2
    Lapin
    Clonalité
    • 41
    • 9
    Polyclonal
    Conjugué
    • 22
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp WNT2B est non-conjugé
    Application
    • 33
    • 13
    • 13
    • 9
    • 9
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Souris, Rat
    Homologie
    Human,Dog,Pig,Horse,Chicken,Rabbit
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human WNT2B
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029638 (Immunohistochemistry)
    'Independent Validation' signe
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029638
    Date
    14.03.2014
    Antigène
    Numéro du lot
    120522
    Application validée
    Immunohistochemistry
    Contrôle positif
    Human kidney
    Contrôle négative
    Human liver
    Conclusion
    Faint but distinguishable signal was detected in positive control sample and not in negative control sample.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antigen: Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (N-Term)
    • Catalog number: ABIN675728
    • Lot number: 120522
    Anticorps secondaire
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D07640BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 min
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
    • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
    • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 5. Slides rinsed with *reaction buffer* x3
    • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
    • 7. Slides rinsed with *reaction buffer*
    • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
    • 9. Slides rinsed with *reaction buffer* x3
    • 10. *iView Biotinylated IgG* applied and incubated for 8 min
    • 11. Slides rinsed with *reaction buffer*
    • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
    • 15. Slides rinsed with *reaction buffer*
    • 16. *iView DAB/H2O2* applied and incubated for 8 min
    • 17. Slides rinsed with *reaction buffer*
    • 18. *iView Copper* applied and incubated for 4 min
    • 19. Slides rinsed with *reaction buffer*
    • 20. Slides washed in Dawn Detergent/tap water
    • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
    • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
    • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Notes
    • Deviations from protocol/procedure supplied by manufacturer (attached).
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
    • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
    • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Li, Liu, Su, Ren, Zhu, Tian, Qiu: "MicroRNA-324-3p regulates nasopharyngeal carcinoma radioresistance by directly targeting WNT2B." dans: European journal of cancer (Oxford, England : 1990), Vol. 49, Issue 11, pp. 2596-607, (2014) (PubMed).

  • Antigène
    WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))
    Autre désignation
    WNT2B (WNT2B Produits)
    Synonymes
    anticorps WNT13, anticorps XWNT2, anticorps wnt2b, anticorps XWnt-2, anticorps Xwnt-2b, anticorps wnt-2b, anticorps xwnt2b, anticorps WNT2B, anticorps Wnt13, anticorps Wnt family member 2B, anticorps wingless-type MMTV integration site family, member 2Ba, anticorps Wnt family member 2B L homeolog, anticorps wingless-type MMTV integration site family, member 2B, anticorps wingless-type MMTV integration site family, member 2Bb, anticorps WNT2B, anticorps Wnt2b, anticorps wnt2ba, anticorps wnt2b.L, anticorps wnt2bb
    Sujet

    Synonyms: WNT13, Protein Wnt-2b, Protein Wnt-13, WNT2B

    Background: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

    ID gène
    7482
    UniProt
    Q93097
    Pathways
    Signalisation WNT
Support technique