MTOR anticorps (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492)

Details for Product anti-FRAP1 Antibody No. ABIN676403, Fournisseur: Connectez-vous pour afficher
Antigène
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Alternatives
anti-Humain MTOR anticorps pour Immunofluorescence (fixed cells)
Épitope
AA 2436-2492
64
28
26
22
21
20
15
14
12
10
9
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
368
200
198
18
9
8
7
7
7
7
4
2
2
2
1
1
Hôte
Lapin
316
48
3
1
Clonalité
Polyclonal
Conjugué
Cet anticorp MTOR est non-conjugé
8
8
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
247
111
94
72
55
51
37
20
7
7
7
6
3
2
2
2
1
1
1
Options
Fournisseur
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Images

'Independent Validation' signe
Numéro du lot 131209
Application validée Western Blotting
Contrôle positif HeLa cells
Contrôle négative c6/36 mosquito cells (non-reactive species)
Conclusion A strong band was observed in the positive control sample at the correct molecular weight. Multiple faint bands were also observed in the positive sample, but these bands did not represent a significant background signal. A band at the correct molecular weight was also observed in the negative control; however, it may be impossible to find a true negative control for this antigen.
Anticorps primaire
Anticorps secondaire
Contrôles
  • Positive control: HeLa cell extract
  • Negative control: c6/36 Mosquito cell extract
Protocole
  • Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
  • The membrane was rinsed with TBST once.
  • The membrane was immersed with the protein side up in the primary antibody solution (FRAP1; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
  • The membrane was rinsed in TBST thrice for 5 min each.
  • The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • The membrane was rinsed thrice TBST thrice for 5 min each.
  • The membrane was rinsed in TBS twice for 30 s each.
  • Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • The membrane was rinsed three times TBST.
  • Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • The membrane was washed in TBST 2 times for 10 min each.
  • Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2436-2492) antibody (ABIN676403) Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) p...
Immunogène KLH conjugated synthetic peptide derived from human mTOR
Isotype IgG
Purification Purified by Protein A.
Autre désignation Mtor/Frap (FRAP1 Antibody Extrait)
Sujet

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Synonyms: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

ID gène 2475
UniProt P42345
Pathways Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events
Indications d'application IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Supplier Images
Immunofluorescence (IF) image for anti-MTOR anticorps (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492) (ABIN676403) Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Pol...
Immunohistochemistry (IHC) image for anti-MTOR anticorps (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492) (ABIN676403) Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MTOR anticorps (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2436-2492) (ABIN676403) Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Pol...
Produit citée dans: Zou, Geng, Chen, Ren, Liu, Wan, Guo, Wang: "Ranolazine improves oxidative stress and mitochondrial function in the atrium of acetylcholine-CaCl2 induced atrial fibrillation rats." dans: Life sciences, Vol. 156, pp. 7-14, 2016 (PubMed).

Madka, Mohammed, Li, Zhang, Biddick, Patlolla, Lightfoot, Towner, Wu, Steele, Kopelovich, Rao: "Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo." dans: Cancer prevention research (Philadelphia, Pa.), Vol. 9, Issue 1, pp. 53-62, 2016 (PubMed).

Yang, Wang, Wang, Zhang, Zhang, Lu, Wang: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." dans: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, 2015 (PubMed).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." dans: Bioscience reports, Vol. 34, Issue 5, 2014 (PubMed).