p38 anticorps (MAP kinase p38) (pThr180)

Details for Product anti-p38 Antibody No. ABIN678668, Fournisseur: Connectez-vous pour afficher
Antigène
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38
  • p38ALPHA
  • mitogen-activated protein kinase 14
  • MAPK14
Alternatives
anti-Humain p38 anticorps pour Immunofluorescence (fixed cells)
Épitope
pThr180, pTyr182, AA 160-200
79
62
33
20
15
14
12
10
9
8
8
6
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus), Chévre
367
265
262
48
30
29
28
26
26
25
21
21
15
14
12
7
5
3
2
1
1
1
Hôte
Lapin
276
94
Clonalité
Polyclonal
Conjugué
Cet anticorp p38 est non-conjugé
10
10
9
7
7
7
6
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
305
159
153
94
67
54
46
29
27
18
13
10
7
6
6
2
1
1
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
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Images

'Independent Validation' signe
Numéro du lot 140106
Application validée Immunohistochemistry
Contrôle positif Human placenta tissue
Contrôle négative Human tonsil tissue (no literature evidence of phospho-p38 MAPK in tonsil)
Conclusion Signal is detected in positive control tissue and not in negative control tissue.
Anticorps primaire
Contrôle isotypique
Anticorps secondaire
Contrôles
  • Positive control: Human placenta tissue stained with antibody
  • Negative control: Human tonsil tissue stained with antibody
  • Isotype control: Human placenta tissue stained with isotype control
  • Secondary only control: Human placenta tissue stained with secondary antibody only
Protocole
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Notes
  • Deviations from protocol/procedure supplied by manufacturer:
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 1: Human placenta tissue stained with anti-phospho-MAPK14 (brown) and counters...
Immunohistochemistry validation image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 2: Human tonsil tissue stained with anti-phospho-MAPK14 (brown) and countersta...
Immunohistochemistry validation image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 3: Human placenta tissue stained with isotype control antibody (brown) and cou...
Immunohistochemistry validation image for anti-MAP kinase p38 (p38) (AA 160-200), (pThr180), (pTyr182) antibody (ABIN678668) Figure 4: Human placenta tissue stained with secondary antibody only (brown) and coun...
Immunogène KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of(Thr180/Tyr182)
Isotype IgG
Purification Purified by Protein A.
Autre désignation P38 Mapk (p38 Antibody Extrait)
Sujet

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Subcellular location: Cytoplasm, Nucleus

Synonyms: RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha, Stress-activated protein kinase 2a, MAPK14

ID gène 1432
UniProt Q16539
Domaine de recherche Kinases/Phosphatases
Pathways Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Supplier Images
Western Blotting (WB) image for anti-p38 anticorps (MAP kinase p38) (pThr180) (ABIN678668) Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibo...
Western Blotting (WB) image for anti-p38 anticorps (MAP kinase p38) (pThr180) (ABIN678668) Images provided by the Independent Validation Program (badge 29798).Lane one: HeLa ce...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p38 anticorps (MAP kinase p38) (pThr180) (ABIN678668) Images provided the Independent Validation Program (badge number 029662)Formalin-fixe...
Produit citée dans: Iriyama, Hatta, Takei: "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." dans: Cancer medicine, Vol. 5, Issue 11, pp. 3223-3234, 2016 (PubMed).

Li, Yu, Liu, Zhu, Hua: "Expression of miR-34c in response to overexpression of Boule and Stra8 in dairy goat male germ line stem cells (mGSCs)." dans: Cell biochemistry and function, Vol. 31, Issue 4, pp. 281-8, 2013 (PubMed).