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Tissue factor anticorps (AA 32-100)

F3 Reactivité: Humain, Souris, Rat, Porc WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN708086
  • Antigène Voir toutes Tissue factor (F3) Anticorps
    Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
    Épitope
    • 15
    • 10
    • 8
    • 7
    • 6
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 32-100
    Reactivité
    • 93
    • 42
    • 37
    • 19
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    Humain, Souris, Rat, Porc
    Hôte
    • 86
    • 31
    • 4
    • 2
    Lapin
    Clonalité
    • 80
    • 42
    • 1
    Polyclonal
    Conjugué
    • 54
    • 21
    • 10
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Tissue factor est non-conjugé
    Application
    • 93
    • 37
    • 35
    • 32
    • 18
    • 14
    • 13
    • 12
    • 11
    • 8
    • 7
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Humain, Souris, Porc, Rat
    Homologie
    Dog,Cow,Horse,Rabbit,Guinea Pig
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human CD142
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029589 (Immunohistochemistry)
    'Independent Validation' signe
    by
    Reveal Biosciences
    No.
    #029589
    Date
    07.02.2014
    Antigène
    Numéro du lot
    980886W
    Application validée
    Immunohistochemistry
    Contrôle positif
    Mouse lung tissue
    Contrôle négative
    Mouse brain tissue
    Conclusion
    Coagulation Factor III (thromboplastin, Tissue Factor) (F3) staining is observed in positive control tissue and not in negative control tissue.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)
    • Catalog number: ABIN708086
    • Lot number: 120224
    Anticorps secondaire
    • Antibody: Bond Polymer Refine Detection Kit
    • Lot number: 24144
    Full Protocol
    • Immunohistochemistry was performed on a Leica Bond automated immunostainer.
    • Sections were deparaffinized with Novocastra Bond Dewax Solution and rehydrated into Leica Bond Wash Buffer.
    • Sections were heated to 98 °C for 20 minutes in 10 mM citrate buffer pH 9.0 (ER1; Leica) for antigen retrieval.
    • Sections were blocked in 3 % casein plus 0.1 % Triton-X100 for 10 minutes at room temperature.
    • Sections were washed x 3 in Leica Bond Wash Buffer.
    • Sections were incubated with primary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 15 minutes at room temperature.
    • Sections were washed x 3 in Leica Bond Wash Buffer.
    • Sections were incubated with Leica Bond Polymer for 8 minutes at room temperature.
    • Sections were washed x 4 in Leica Bond Wash Buffer.
    • Sections were washed x 1 in Distilled Water.
    • Sections were incubated with Peroxide Block (Leica) for 10 min to block endogenous peroxidase.
    • Sections were washed x 4 in Leica Bond Wash Buffer.
    • Sections were incubated with DAB chromogenic substrate (Leica) for 10 min at RT.
    • Sections were washed x 3 in Distilled Water.
    • Sections were counterstained with hematoxylin (Leica) for 2 minutes.
    • Sections were washed x 1 in Distilled Water.
    • Sections were washed x 1 in Leica Bond Wash Buffer.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated, mounted and photographed under a light microscope.
    Notes
    None notes.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Fletcher, Das, Gadye, Street, Baudhuin, Wagner, Cole, Flores, Choi, Yosef, Purdom, Dudoit, Risso, Ngai: "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." dans: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).

    Kim, Yoo, Gu, Kim: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." dans: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, (2016) (PubMed).

    Yoo, Kim, Gu, Lee, Lee, Hwang, Hwang, Kim, Kim: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." dans: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, (2016) (PubMed).

    Gleeson, Martin, Ali, Kumar, Pillai, Kumar, OSullivan, Whelan, Stocca, Khider, Barry, OBrien, Caplice: "Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy." dans: Stem cells (Dayton, Ohio), (2015) (PubMed).

    Tian, Salsbery, Wang, Yuan, Yang, Zhao, Wu, Zhang, Konkle, Thiagarajan, Li, Zhang, Dong: "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." dans: Blood, (2015) (PubMed).

    Milano, Dongiovanni, Artoni, Gatti, Rosso, Colombo, Bollati, Maggioni, Mannucci, Bertazzi, Fargion, Valenti: "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." dans: Journal of applied toxicology : JAT, (2015) (PubMed).

    Liu, Wei, Zhang, Xu, Wang, Zhou: "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." dans: Thrombosis research, Vol. 133, Issue 4, pp. 645-51, (2014) (PubMed).

  • Antigène
    Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
    Autre désignation
    CD142 (F3 Produits)
    Synonymes
    anticorps CD142, anticorps TF, anticorps TFA, anticorps PRO1557, anticorps PRO2086, anticorps TFQTL1, anticorps f3, anticorps AA409063, anticorps Cf-3, anticorps Cf3, anticorps tf, anticorps zgc:112151, anticorps coagulation factor III, tissue factor, anticorps transferrin, anticorps coagulation factor IIIa, anticorps coagulation factor III, anticorps tissue factor, anticorps coagulation factor IIIb, anticorps coagulation factor III (thromboplastin, tissue factor) S homeolog, anticorps F3, anticorps TF, anticorps f3a, anticorps tf, anticorps f3b, anticorps f3.S
    Sujet

    Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3

    Background: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

    ID gène
    2152
    UniProt
    P13726
    Pathways
    Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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