AKT 1/2/3 anticorps (AA 401-479)

N° du produit ABIN725195

Détail du produit anti-AKT 1/2/3 anticorps

Antigène: AKT 1/2/3

Épitope: AA 401-479

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT 1/2/3 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Boeuf (Vache), Humain, Souris, Porc, Rat

Homologie: Dog,Cow,Sheep,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human AKT1

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029769 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029769

Date: 22.07.2014

Numéro du lot: 131113

Application validée: Flow Cytometry

Contrôle positif: MDA-MB-468 cells - lower expression

Conclusion: A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.

Validation Images

Figure 1: Positive control (high expression) MCF7 cells. The red histogram is unstained cells, the blue histogram is cells stained with secondary antibody alone, the green histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody and the orange histogram is cells stained with anti-AKT1 plus secondary antibody. The secondary antibody is a goat anti-rabbit IgG-FITC (Jackson Immunoresearch).

Figure 2: Positive control (lower expression) MDA-MB-468 cells. The red histogram is unstained cells, the blue histogram is cells stained with secondary antibody alone, the green histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody and the orange histogram is cells stained with anti-AKT1 plus secondary antibody. The secondary antibody is a goat anti-rabbit IgG-FITC (Jackson Immunoresearch).

Protocole

Anticorps primaire:

• Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
• Catalog number: ABIN725195
• Lot number: 131113
• Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA

Anticorps secondaire:

• Antibody: Goat anti-rabbit IgG-FITC
• Lot number: 115629
• Dilution: 1:200 in 1X PBS containing 0.5% BSA

Full Protocol:

• Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
• Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
• An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
• Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
• Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
• Cells were incubated for 30 min on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
• Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).

Notes: Nothing to note.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-AKT 1/2/3 (ABIN725195)

Liu, Liu, Liu, Ma, Zhao: "Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala." dans: Molecular pain, Vol. 14, pp. 1744806918805902, (2019) (PubMed).

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." dans: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." dans: Scientific reports, Vol. 6, pp. 37884, (2016) (PubMed).

Zhang, Huang, Guo, Gou, Hinsdale, Lloyd, Liu: "MicroRNA-26b Modulates the NF-κB Pathway in Alveolar Macrophages by Regulating PTEN." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 11, pp. 5404-14, (2015) (PubMed).

Liang, Zeng, Wang, Shen, Li, Ma, Ci, Yu, Jia, Sun, Liu, Liu, Li, Yu, Chen, Jia: "Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells." dans: Oncotarget, Vol. 5, Issue 14, pp. 5798-807, (2015) (PubMed).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." dans: Bioscience reports, Vol. 34, Issue 5, (2014) (PubMed).

Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." dans: BMC cancer, Vol. 13, pp. 247, (2013) (PubMed).

Tang, Liu, Dong, Li, Li, Lin, Zheng, Hou, Li: "The cardioprotective effects of citric Acid and L-malic Acid on myocardial ischemia/reperfusion injury." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 820695, (2013) (PubMed).

Détails sur AKT 1/2/3

Antigène: AKT 1/2/3

Autre désignation: AKT1/2/3 (AKT 1/2/3 Produits)

Sujet:

Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG

Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

ID gène: 207

UniProt: P31749