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TTF1 anticorps (AA 201-300)

TTF1 Reactivité: Humain, Rat, Souris WB, FACS, IF (cc), ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN728713
  • Antigène Voir toutes TTF1 Anticorps
    TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
    Épitope
    • 15
    • 7
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 201-300
    Reactivité
    Humain, Rat, Souris
    Hôte
    • 47
    • 8
    Lapin
    Clonalité
    • 44
    • 11
    Polyclonal
    Conjugué
    • 26
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TTF1 est non-conjugé
    Application
    • 32
    • 20
    • 15
    • 12
    • 10
    • 7
    • 6
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC)
     Réactivité croisée
    Humain, Souris, Rat
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human TTF-1
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Validation #028752 (Western Blotting)
    'Independent Validation' signe
    by
    Alamo Laboratories Inc
    No.
    #028752
    Date
    08.09.2013
    Antigène
    Numéro du lot
    120319
    Application validée
    Western Blotting
    Contrôle positif
    Brain
    Contrôle négative
    Liver
    Conclusion
    A strong band was observed at the expected size in the positive control lysate but not in the negative control lysate.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antibody: NK2 Homeobox 1 (NKX2-1) antibody
    • Catalog number: ABIN728713
    • Lot number: 120319
    Anticorps secondaire
    • Antibody: Goat anti-Rabbit IgG Antibody (HRP)
    • Lot number: YYDW62W
    Full Protocol
    • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% Beta-mercaptoethanol at 95°C for 5 minutes prior to loading.
    • 2. 24 µg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
    • 3. The Spectra Multicolor Broad Range molecular mass marker (26634 Thermo Scientific) was used as a standard.
    • 4. Proteins were transferred onto PVDF membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
    • 5. The immunoblot membrane was blocked in PBS containing 3% (W/V) non-fat dry milk at room temperature for 1 hour.
    • 6. The membrane was rinsed with PBS containing 0.05% Tween-20 once.
    • 7. The membrane was immersed with the protein side up in the antibody solution in PBS containing 1% (W/V) non-fat dry milk and incubated for 2 hours at room temperature (~26°C).
    • 8. The membrane was rinsed in PBS containing 0.05% Tween-20 thrice for 10 min each.
    • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in PBS containing 1% (W/V) non-fat dry milk and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
    • 10. The membrane was rinsed in PBS containing 0.05% Tween-20 thrice for 10 min each.
    • 11. The membrane was washed in PBS twice for 30 seconds each.
    • 12. Signals were detected with Pierce ECL Western Blotting Substrate (32109, Thermo Scientific). The blot was scanned for 300 seconds.
    • 13. The membrane was rinsed three times with PBS containing 0.05% Tween-20.
    • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
    • 15. The membrane was washed in PBS containing 0.05% Tween-20 times for 10 min each.
    • 16. Repeated Steps 5-12 with the loading control antibody (for Beta-actin) and its matching secondary antibody.
    Notes
    None
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Vadasz, Jensen, Moncada, Girard, Zhang, Blanchette, Finck: "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." dans: Journal of pediatric surgery, Vol. 49, Issue 11, pp. 1554-63, (2014) (PubMed).

    Huang, Li, Lin, Shi, Lin, Li, Xu: "Upregulation of thyroid transcription factor-1 and human leukocyte antigen class I in Hashimoto's disease providing a clinical evidence for possible triggering autoimmune reaction." dans: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 164, Issue 5, pp. 795-800, (2011) (PubMed).

  • Antigène
    TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
    Autre désignation
    TTF1 (TTF1 Produits)
    Synonymes
    anticorps TTF-1, anticorps TTF-I, anticorps AV245725, anticorps RGD1565673, anticorps transcription termination factor 1, anticorps transcription termination factor, RNA polymerase I, anticorps TTF1, anticorps Ttf1
    Sujet

    Synonyms: BCH, BHC, NK-2, TEBP, TTF1, NKX2A, T/EBP, TITF1, TTF-1, NKX2.1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, NKX2-1

    Background: Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).

    ID gène
    7080
    UniProt
    P43699
    Pathways
    Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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