Elastin anticorps (AA 681-786)

N° du produit ABIN734003

Détail du produit anti-Elastin anticorps

Antigène: Elastin (ELN)

Épitope: AA 681-786

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Elastin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Elastin

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #028751 (Immunohistochemistry)

by: Beth Israel Deaconess Medical Center Confocal Imaging Core

No.: #028751

Date: 25.09.2013

Numéro du lot: 120306

Application validée: Immunohistochemistry

Contrôle positif: Mouse heart tissue

Contrôle négative: Mouse adipose tissue

Conclusion: Signal was detected in positive control tissue and not detected in negative control tissue.

Validation Images

Figure 1: Immunostaining of Elastin antibody. Panel 1: Staining of mouse heart tissue stained with anti-Elastin antibody (brown). Panel 2: Staining of mouse adipose tissue with anti-Elastin antibody. Panel 3: Staining of mouse heart tissue with isotype control antibody. Panel 4: Staining of mouse heart tissue with secondary antibody only. All panels are counterstained with hematoxylin (blue).

Protocole

Anticorps primaire:

• Antibody: Elastin (ELN) (C-Term) antibody
• Catalog number: ABIN734003
• Lot number: 120306

Anticorps secondaire:

• Antibody: Donkey anti-Rabbit IgG Antibody (Biotinylated)

Full Protocol:

• Immunohistochemistry was performed manually.
• Sections were deparaffinized and rehydrated.
• Sections were heated to 98°C for 10 min in 10 mM citrate buffer pH 6.0 for antigen retrieval.
• Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidase.
• Sections were washed x 3 in Tris buffered saline (TBS).
• Sections were blocked in 5% normal donkey serum for 60 min at room temperature.
• Sections were incubated with primary antibody diluted 1:1000 in 5% normal donkey serum in TBS. Incubated at 4°C overnight.
• Sections were washed x 3 in Tris buffered saline.
• Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature.
• Sections were washed x 3 in Tris buffered saline.
• Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
• Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 seconds at room temperature.
• Sections were washed x 3 in Distilled Water.
• Sections were counterstained with hematoxylin for 1 min.
• Sections were washed x 1 in Distilled Water.
• Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.

Notes: None

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-Elastin (ABIN734003)

Hasegawa, Sato, Shirai, Watanabe, Yamamoto, Watanabe, Nohtomi, Hirano, Watanabe: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." dans: PLoS ONE, Vol. 9, Issue 12, pp. e110866, (2014) (PubMed).

Konii, Sato, Kikuchi, Okiyama, Watanabe, Hasegawa, Yamamoto, Itoh, Hirano, Watanabe: "Stimulatory effects of cardiotrophin 1 on atherosclerosis." dans: Hypertension, Vol. 62, Issue 5, pp. 942-50, (2013) (PubMed).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." dans: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, (2012) (PubMed).

Ikeda, Yada, Takase: "[Syntheses of 1,3,4-thiadiazole derivatives]." dans: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Vol. 90, Issue 1, pp. 95-8, (1970) (PubMed).

Détails sur Elastin

Antigène: Elastin (ELN)

Autre désignation: Elastin (ELN Produits)

Synonymes: anticorps SVAS, anticorps WBS, anticorps WS, anticorps AI385707, anticorps AI480567, anticorps E030024M20Rik, anticorps RATTREL11, anticorps TREL11, anticorps Trela, anticorps Trela26, anticorps ELN, anticorps elastin, anticorps ELN, anticorps Eln, anticorps LOC574132, anticorps Tcur_2481, anticorps Kfla_2289, anticorps LOC100620140

Sujet:

Synonyms: WS, WBS, SVAS, Elastin, Tropoelastin, ELN

Background: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

ID gène: 2006

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization