Osteoactivin (AA 85-135) anticorps

Détails pour le produit réf. ABIN749093, Fournisseur: Connectez-vous pour afficher
Antigène
  • HGFIN
  • NMB
  • DC-HIL
  • Dchil
  • ipd
  • glycoprotein (transmembrane) nmb
  • Gpnmb
  • GPNMB
  • gpnmb
Épitope
AA 85-135
19
18
14
11
9
8
8
7
5
4
4
3
2
2
2
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
121
28
23
5
5
5
4
4
2
2
2
1
1
Hôte
Lapin
68
58
1
Clonalité
Polyclonal
Conjugué
Inconjugué
5
5
4
4
4
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
77
59
37
21
15
12
4
3
3
1
Options
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Images

'Independent Validation' signe
Antigène Osteoactivin
Application validée Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
Contrôle positif Tumor cell transplanted mouse brain was dissected
Conclusion The staining pattern was as expected for Osteoactivin as membrane protein.
Anticorps primaire ABIN749093
Contrôle isotypique rabbit IgG
Anticorps secondaire Donkey-anti-Rabbit-Cy3
Protocole
  • Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
  • Immunostaining for Cryo Sections:
    • Dry slides at 50-55°C for 15-30 min (or RT for 30min)
    • Wash three times with PBS 5min (shake gently)
    • Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
    • Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
    • Rabbit-anti-Osteoactivin antibody (ABIN749093) diluted 1:50-1:400
    • Wash three times with PBS 5min (shake gently)
    • Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
    • Donkey-anti-Rabbit-Cy3
    • DAPI 1:100
    • Wash three times with PBS 5min (shake gently)
    • Add three drops of water-based Immu-mount
    • Cover with 24x60 cover glass
  • Imaging
    • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
    Notes ABIN749093 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.
    Validation Images
    Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) validation image for anti-Osteoactivin (AA 85-135) antibody (ABIN749093) IF validation of osteoactivin antibody ABIN749093 on mouse brain tumor sections. A. A...
    Immunogène KLH conjugated synthetic peptide derived from human GPNMB
    Isotype IgG
    Purification Purified by Protein A.
    Autre désignation Gpnmb
    Sujet

    The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

    Subcellular location: Extracellular

    Synonyms: NMB, HGFIN, Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, UNQ1725/PRO9925

    ID gène 10457
    UniProt Q14956
    Domaine de recherche Cancer, Innate Immunity
    Indications d'application WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    Restrictions For Research Use only
    Format Liquid
    Concentration 1 μg/μL
    Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
    Agent conservateur Sodium azide
    Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock -20 °C
    Stockage commentaire Store at -20°C
    Date de péremption 12 months
    Supplier Images
    SDS-PAGE (SDS) image for anti-Osteoactivin (AA 85-135) anticorps (ABIN749093) Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Po...
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Osteoactivin (AA 85-135) anticorps (ABIN749093) Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Po...
    Produit citée dans: Arosarena, Dela Cadena, Denny, Bryant, Barr, Thorpe, Safadi: "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." dans: Journal of cellular physiology, Vol. 231, Issue 8, pp. 1761-70, 2016 (PubMed).

    Frara, Abdelmagid, Tytell, Amin, Popoff, Safadi, Barbe: "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." dans: BMC musculoskeletal disorders, Vol. 17, pp. 34, 2016 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)).

    Ruan, Li, Nie, Zhou, Zou: "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." dans: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 4857-66, 2015 (PubMed). Méthode utilisée: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Échantillon (espèces): Human).

    Abdelmagid, Sondag, Moussa, Belcher, Yu, Stinnett, Novak, Mbimba, Khol, Hankenson, Malcuit, Safadi: "Mutation in Osteoactivin Promotes Receptor Activator of NF?B Ligand (RANKL)-mediated Osteoclast Differentiation and Survival but Inhibits Osteoclast Function." dans: The Journal of biological chemistry, Vol. 290, Issue 33, pp. 20128-46, 2015 (PubMed).