DARPP32 anticorps (pSer137)

N° du produit ABIN784599

Détail du produit anti-DARPP32 anticorps

Antigène: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Épitope: pSer137

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Poulet, Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DARPP32 est non-conjugé

Application: Western Blotting (WB)

Specificité: Specific for the ~32k DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by by preadsorption with the phospho-peptide used as antigen but not by the dephospho-peptide.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser137 of Rat DARPP-32.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -20 °C.

Détails sur DARPP32

Antigène: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Autre désignation: DARPP32 (PPP1R1B Produits)

Synonymes: anticorps darpp32, anticorps darpp-32, anticorps Darpp-32, anticorps Darpp32, anticorps DARPP-32, anticorps DARPP32, anticorps AU040756, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 1B, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog, anticorps ppp1r1b, anticorps PPP1R1B, anticorps Ppp1r1b, anticorps ppp1r1b.S

Sujet: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Serine 137 is phosphorylated by casein kinase 1 and phosphorylation of this site is increased following acute administration of Prozac® (Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B

ID gène: 84152

NCBI Accession: NP_001229393