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HIF1A anticorps (AA 329-530)

HIF1A Reactivité: Souris WB, IHC, ELISA, IF, ICC Hôte: Souris Monoclonal ESEE122 unconjugated
N° du produit ABIN863096
  • Antigène Voir toutes HIF1A Anticorps
    HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
    Épitope
    • 21
    • 19
    • 16
    • 16
    • 12
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 329-530
    Reactivité
    • 173
    • 86
    • 81
    • 24
    • 23
    • 20
    • 14
    • 12
    • 9
    • 8
    • 7
    • 6
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 160
    • 58
    • 6
    Souris
    Clonalité
    • 166
    • 58
    Monoclonal
    Conjugué
    • 118
    • 29
    • 18
    • 8
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HIF1A est non-conjugé
    Application
    • 191
    • 92
    • 76
    • 59
    • 54
    • 40
    • 40
    • 27
    • 26
    • 26
    • 12
    • 9
    • 7
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Detects ~116 kDa. Specific for HIF1Alpha.
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Rat
    Purification
    Protein G Purified
    Immunogène
    Recombinant fragment corresponding to amino acids 329-530
    Clone
    ESEE122
    Isotype
    IgG1
    Top Product
    Discover our top product HIF1A Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:50)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml of ABIN863096 was sufficient for detection of HIF1α in 20 μg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Surazynski, Miltyk, Prokop, Palka: "The effect of estrogen on prolidase-dependent regulation of HIF-1? expression in breast cancer cells." dans: Molecular and cellular biochemistry, Vol. 379, Issue 1-2, pp. 29-36, (2013) (PubMed).

  • Antigène
    HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
    Autre désignation
    HIF 1 alpha (HIF1A Produits)
    Synonymes
    anticorps hif1a, anticorps AA959795, anticorps HIF1alpha, anticorps MOP1, anticorps bHLHe78, anticorps HIF-1A, anticorps HIF-1alpha, anticorps HIF1, anticorps HIF1-ALPHA, anticorps PASD8, anticorps HIF1-alpha, anticorps hif-1a, anticorps hypoxia inducible factor 1 alpha, anticorps hypoxia inducible factor 1, alpha subunit, anticorps hypoxia inducible factor 1 alpha subunit, anticorps HIF (Hypoxia Inducible Factor) homologa, anticorps hif-1a, anticorps Hif1a, anticorps HIF1A, anticorps hif1a, anticorps hif-1
    Sujet
    Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex, ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
    ID gène
    15251
    NCBI Accession
    NP_034561
    UniProt
    Q61221
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg
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