HSPB8 anticorps
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- Antigène Voir toutes HSPB8 Anticorps
- HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp HSPB8 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificité
- Detects ~22 kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.
- Réactivité croisée
- Humain, Souris, Rat
- Purification
- Protein G Purified
- Immunogène
- His-tagged human recombinant HSP22
- Clone
- 3C12-H11
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product HSPB8 Anticorps primaire
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- Indications d'application
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- WB (1:2000)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
- Commentaires
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1 μg/ml of ABIN863102 was sufficient for detection of HSP22 in 20 μg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- -20°C
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- Antigène
- HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))
- Autre désignation
- HSP22 (HSPB8 Produits)
- Synonymes
- anticorps CMT2L, anticorps DHMN2, anticorps E2IG1, anticorps H11, anticorps HMN2, anticorps HMN2A, anticorps HSP22, anticorps MGC64408, anticorps hsc70, anticorps wu:fb01g06, anticorps wu:fi48b06, anticorps fc09c11, anticorps wu:fc04b04, anticorps wu:fc09c11, anticorps zgc:64202, anticorps AU018630, anticorps AW413033, anticorps Cryac, anticorps D5Ucla4, anticorps H11K, anticorps HSP20-like, anticorps Hsp22, anticorps heat shock protein family B (small) member 8, anticorps heat shock protein family B (small) member 8 L homeolog, anticorps heat shock protein 8, anticorps heat shock protein b8, anticorps HSPB8, anticorps hspb8.L, anticorps hspa8, anticorps hspb8, anticorps Hspb8
- Sujet
- HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6).
- ID gène
- 26353
- NCBI Accession
- NP_055180
- UniProt
- Q9UJY1
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