Lhca1 anticorps

N° du produit ABIN5684014

Détail du produit anti-Lhca1 anticorps

Antigène: Lhca1 (psi Type I Chlorophyll A/B-Binding Protein (Lhca1))

Reactivité: Arabidopsis thaliana, Orge, Green bean, Guzmania hybrid, Moss, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinach, Zea mays

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB)

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 22 / 22 kDa for Arabidopsis thaliana

Purification: affinity purified

Immunogène: BSA-conjugated synthetic peptide derived from the Lhca1 protein of Arabidopsis thaliana UniProt: Q01667, TAIR: At3g54890. This sequence is highly conserved in Lhca1 proteins of angiosperms (monocots and dicots) and gymnosperms.

Isotype: IgG

Information d'application

Indications d'application: Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).

Commentaires:

A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA freepeptide, indicating that all bands are indeed PsbA and its precursors or breakdown product.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Références pour anticorps anti-Lhca1 (ABIN5684014)

Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." dans: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

Sakuraba, Rahman, Cho, Kim, Koh, Yoo, Paek: "The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions." dans: The Plant journal : for cell and molecular biology, Vol. 74, Issue 1, pp. 122-33, (2013) (PubMed).

Aubry, Mani, Hoertensteiner: "Stay-green protein, defective in Mendel's green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway." dans: Plant molecular biology, Vol. 67, Issue 3, pp. 243-56, (2008) (PubMed).

Détails sur Lhca1

Antigène: Lhca1 (psi Type I Chlorophyll A/B-Binding Protein (Lhca1))

Sujet: AGI Code: At3g54890
The light-harvesting protein Lhca1 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca1 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca1 forms a heterodimer (LHCI-730) with Lhca4 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073

Poids moléculaire: expected: 22 kDa, apparent: 22 kDa for Arabidopsis thaliana

UniProt: Q01667