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RBCL (Isoform 1) anticorps

Reactivité: Arabidopsis thaliana, Lobaria pulmonaria, Pisum sativum, Potato, Spinach WB, IL Hôte: Poulet Polyclonal unconjugated
N° du produit ABIN93524
  • Antigène
    RBCL
    Épitope
    • 8
    • 4
    • 1
    • 1
    • 1
    Isoform 1
    Reactivité
    Arabidopsis thaliana, Lobaria pulmonaria, Pisum sativum, Potato, Spinach
    Hôte
    • 14
    Poulet
    Clonalité
    • 14
    Polyclonal
    Conjugué
    • 5
    • 3
    • 3
    • 3
    Inconjugué
    Application
    • 10
    • 5
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunolocalization (IL)
    Attributs du produit
    Expected / apparent Molecular Weight of the Antigene: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
    Purification
    affinity purified
    Immunogène
    KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1
  • Indications d'application
    Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 2 µg of total cellular protein, detected with standard ECL(WB), (IL) tested on a grass species, formaldehyde-fixed and paraffin-embeddedtissue following the protocol from Gonzalez et al. (1998) Plant Physiol. V. 116.
    Commentaires

    This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For reconstitution add 225 µL of milliQ water per each tube
    Concentration
    16 µg/µL
    Buffer
    PBS pH 8.0+ 0.02 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Make aliquots to avoid working with a stock.
    Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubek.
    Stock
    4 °C
  • Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." dans: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, (2008) (PubMed).

    Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." dans: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

    Menon, Dou, Heinhorst, Shively, Cannon: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." dans: PLoS ONE, Vol. 3, Issue 10, pp. e3570, (2008) (PubMed).

  • Antigène
    RBCL
    Sujet
    AGI Code: ATCG00490
    Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
    Poids moléculaire
    52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
    UniProt
    Q3ALL1, O03042
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