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PAG1 anticorps (C-Term)

PAG1 Reactivité: Humain, Souris, Rat, Boeuf (Vache) WB, IP, IHC (p) Hôte: Souris Monoclonal PAG-C1 unconjugated
N° du produit ABIN94270
  • Antigène Voir toutes PAG1 Anticorps
    PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
    Épitope
    • 16
    • 10
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 27
    • 26
    • 23
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Boeuf (Vache)
    Hôte
    • 43
    • 3
    Souris
    Clonalité
    • 43
    • 3
    Monoclonal
    Conjugué
    • 18
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PAG1 est non-conjugé
    Application
    • 33
    • 16
    • 13
    • 13
    • 12
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificité
    The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
    Réactivité croisée (Details)
    Mouse, Human, Rat, Bovine, Other not tested
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
    Clone
    PAG-C1
    Isotype
    IgG2b
    Top Product
    Discover our top product PAG1 Anticorps primaire
  • Indications d'application
    Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1.
    Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." dans: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, (2003) (PubMed).

    Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." dans: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, (2003) (PubMed).

  • Antigène
    PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
    Autre désignation
    PAG / Cbp (PAG1 Produits)
    Synonymes
    anticorps Cbp, anticorps F730007C19Rik, anticorps Pag, anticorps CBP, anticorps PAG, anticorps MGC80192, anticorps phosphoprotein associated with glycosphingolipid microdomains 1, anticorps phosphoprotein membrane anchor with glycosphingolipid microdomains 1, anticorps phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog, anticorps Pag1, anticorps PAG1, anticorps pag1.L, anticorps pag1
    Sujet
    Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG
    ID gène
    94212
    UniProt
    Q3U1F9
    Pathways
    Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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