Souris anti-Humain IgM (Fc Region) Anticorps (FITC)

Détails pour le produit réf. ABIN94403, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Fc Region
584
373
290
63
56
51
45
28
6
6
2
1
1
1
1
Reactivité
Humain
1232
574
236
91
80
67
67
58
56
56
56
40
35
28
19
15
15
7
5
5
2
1
1
1
Hôte
Souris
1292
679
527
144
38
11
10
9
4
3
3
1
Clonalité (Clone)
Monoclonal ()
Conjugué
FITC
359
344
271
157
98
74
67
53
25
25
23
22
22
20
20
19
18
18
16
16
15
14
13
12
9
7
7
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
Options
Fournisseur
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Supplier Product No.
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Immunogène Purified human IgM.
Clone CH2
Isotype IgG
Specificité The antibody CH2 reacts with Fc fragment of human IgM.
Attributs du produit The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Purification Purified from rabbit serum by protein-G affinity chromatography.
Sujet Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Poids moléculaire 900 kDa
Domaine de recherche Secondary Antibodies
Indications d'application The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1: 500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Commentaires

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation Do not freeze.
Avoid prolonged exposure to light.
Stock 4 °C
Stockage commentaire Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Flow Cytometry (FACS) image for Mouse anti-Human IgM (Fc Region) antibody (FITC) (ABIN94403) Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." dans: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." dans: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." dans: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." dans: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).