Souris anti-Humain IgM (Fc Region) Anticorps (FITC)

Détails pour le produit réf. ABIN94403, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Fc Region
558
373
290
63
56
51
46
28
6
6
2
1
1
1
1
Reactivité
Humain
1191
559
232
92
80
67
66
58
56
56
56
40
35
28
19
15
15
7
5
5
2
1
1
1
Hôte
Souris
1262
665
511
143
38
11
10
9
4
3
3
2
Clonalité (Clone)
Monoclonal ()
Conjugué
FITC
357
342
271
157
99
74
67
53
25
25
23
22
22
19
18
16
16
16
16
15
14
14
13
12
9
7
7
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
Options
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
Connectez-vous pour afficher
Request

Ce produit gratuit

Envoyez-nous votre proposition de validation. J'aimerais valider ce produit

Savoir plus

Images

Immunogène Purified human IgM.
Clone CH2
Isotype IgG
Specificité The antibody CH2 reacts with Fc fragment of human IgM.
Attributs du produit The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Purification Purified from rabbit serum by protein-G affinity chromatography.
Sujet Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Poids moléculaire 900 kDa
Domaine de recherche Secondary Antibodies
Indications d'application The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1: 500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Commentaires

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation Do not freeze.
Avoid prolonged exposure to light.
Stock 4 °C
Stockage commentaire Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Flow Cytometry (FACS) image for Souris anti-Humain IgM (Fc Region) Anticorps (FITC) (ABIN94403) Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." dans: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." dans: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." dans: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." dans: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).