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Phosphotyrosine anticorps

Name: Phosphotyrosine anticorps
Brand: antibodies-online
SKU: ABIN94452
Price: 380.66 EUR
Availability: InStock

Reactivité: Différentes espèces WB, FACS, ICC Hôte: Souris Monoclonal P-Tyr-01 unconjugated

Anti-Phosphotyrosine purified antibody (clone P-Tyr-01) Specificity Verification by Flow Cytometry Anti-Phosphotyrosine purified antibody (concentration in sample 2 μg/mL, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel).

FACS

Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor ® 488 (green).

2 images

(3 références)

N° du produit ABIN94452

Fiche de données

Antigène Tous les produits Phosphotyrosine

Phosphotyrosine
Reactivité
19

16

16

4

1
Différentes espèces
Hôte
96

28

1
Souris
Anticorps secondaires appropriés
Clonalité
108

17
Monoclonal
Conjugué
44

16

12

8

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp Phosphotyrosine est non-conjugé
Application
88

65

42

41

40

27

24

17

13

9

9

7

6

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificité

The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Réactivité croisée (Details)

Broad

Purification

Purified by protein-A affinity chromatography.

Pureté

> 95 % (by SDS-PAGE)

Immunogène

Phosphotyrosine conjugated to bovine serum albumin.

Clone

P-Tyr-01

Isotype

IgG1
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Hôte: Lapin Polyclonal unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, FACS, ELISA, ICC Hôte: Souris Monoclonal E10 unconjugated

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ELISA, ICC Hôte: Lapin Polyclonal FITC

anti-Phosphotyrosine antibody (Atto 488)
WB, IP, IF, IHC, ELISA, ICC Hôte: Lapin Polyclonal Atto 488

anti-Phosphotyrosine antibody (Atto 594)
WB, IP, IF, IHC, ELISA, ICC Hôte: Lapin Polyclonal Atto 594

anti-Phosphotyrosine antibody (Atto 390)
WB, IP, IF, IHC, ELISA, ICC Hôte: Lapin Polyclonal Atto 390

anti-Phosphotyrosine antibody
WB, IP, IHC, FACS, ELISA, ICC Hôte: Lapin Monoclonal RM111 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Hôte: Souris Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivité: Différentes espèces WB, IHC (p) Hôte: Souris Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivité: Différentes espèces WB, IHC (p) Hôte: Souris Monoclonal 3F6 unconjugated

Indications d'application

Western blotting: Recommended dilution: 1-2 μg/mL. Flow cytometry: Recommended dilution: 2-5 μg/mL. Intracellular staining.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Do not freeze.

Stock

4 °C

Stockage commentaire

Store at 2-8°C. Do not freeze.
Biebl, Riedl, Buchner: "Hsp90 Co-chaperones Form Plastic Genetic Networks Adapted to Client Maturation." dans: Cell reports, Vol. 32, Issue 8, pp. 108063, (2020) (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." dans: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).
Antigène

Phosphotyrosine

Abstract

Phosphotyrosine Produits

Classe de substances

Amino Acid

Sujet

PY