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Glycine Amidinotransferase (L-Arginine:glycine Amidinotransferase) (GATM) anticorps

Détails pour le produit réf. ABIN95359, Fournisseur: Connectez-vous pour afficher Nouveau
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Antigène
Synonymes AT, AGAT, CCDS3, 1810003P21Rik, AI314789, cb409, wu:fa08a06, zgc:65855
Reactivité
Amino Acid
(77), (24), (23), (2), (1), (1), (1), (1), (1), (1)
Hôte
Lapin
(68), (12)
Clonalité
Polyclonal
Conjugué
Inconjugué
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochimie (ICC)
(62), (39), (26), (11), (9), (6), (5), (2), (2), (1), (1), (1)
Pubmed 2 références disponible
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Numéro du produit fournisseur Connectez-vous pour afficher Nouveau
Quantité 100 μL
Destination France ( )
Disponibilité Envoi sous 2 à 3 jours ouvrables
Immunogène Synthetic L-arginine conjugated to protein carrier (Pc)
Isotype IgG
Specificité Conjugated L-arginine . Using a conjugate L-arginine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compound Cross-reactivity ratio (a) Arginine-G-(Pc) 1 Glutamine-G-(Pc) 1/> 100,000 Asparagine-G-(Pc) 1/> 100,000 Lysine-G-(Pc) 1/> 100,000 (a): Arginine-G-(Pc) concentration / other conjugated aminoacids concentration at half displacement G = Glutaraldehyde
Purification Antiserum previously preabsorbed on protein carriers, and purified.
Autre désignation Arginine, L (GATM Antibody Extrait)
Classe de substances Amino Acid
Domaine de recherche Hormones, Receptors
Indications d'application Example of cytochemistry application Perfusion protocol for adult male sprague dawley (weight around 0.5kg):1- The animals can be deeply anesthetized with for example urethane (0.5-1.5g/kg, intraperitoneal). 2- Heparinized, and perfused via the ascending aorta with 100ml of cold physiologic saline (0.9% NaCl) and with the following fixative solution:a) 300ml of cold 4% paraformaldehyde and 2% glutaraldehyde in 0.1M phosphate-buffer (PB), pH 7.2 (two minutes). b) 600ml of cold 4% paraformaldehyde and 2% glutaraldehyde in 0.1M phosphate-buffer (PB), pH 7.2 (ten minutes). c) Dissect out the brains and place in a solution of 4% paraformaldehyde in 0.1M PB, pH 7.2, at 4ºC for twelve to sixteen hours. d) Before the brains will be cut on a freezing microtome, we must include the brain in growing concentrations of sucrose (a first bain of 5% of sucrose in PBS until the brains sank), after that we will repeat the same process in a solution with a higher level of sucrose (10%), 20%, 25% and finally 30%. Around 50 µm-thick serial sections will be obtained, kept at 4º C in PBS (0.1M, pH 7.2) and processed for immunostaining. Example of immunohistochemical protocol 1- In order to avoid possible interference with endogenous peroxidase, free-floating sections will be treated with distilled water containing NH 3 (20%), H 2 O 2 (30%) and NaOH (1%) for 20 min (other method is using a solution with 33% of H 2 O 2 and 66% of methanol). 2- Then, wash the sections for 20 min in 0.15M phosphate-buffered saline (PBS) (pH 7.2) 3- Pre-incubate for 30 min in PBS containing 10% of normal horse serum and 0.3% of Triton X-100 (mixed solution). 4- Incubate at room temperature (1h30min) and overnight at 4ºC in the same mixed solution containing L-Arginine antiserum (diluted as recommended dilution). 5- Then, the sections will be wash in PBS (30 min). 6- After that we will incubate for 60 min at room temperature with biotinylated anti-rabbit immunogammaglobulin (Vector) diluted 1/200 in PBS. 7- Wash during 30 min with PBS. 8- Sections will be incubated for 1h with a 1/100 diluted avidin-biotin-peroxidase complex (Vectastain). 9- After that we will wash the sections in PBS (30 min) 10- Wash with Tris-HCl buffer (pH 7.6)(10 min). 11- The tissue-bound peroxidase will be developed with H 2 O 2 using 3, 3'-diaminobenzidine as chromogen. 12- Finally the sections will be rinsed with PBS and coverslipped with PBS/Glycerol (1/1). Gemacbio sells the anti-conjugated Citrulline and anti-conjugated Agmatine antibodies raised in rabbit: used together, these tools could be helpful for immunocytochemistry double labelling. Recommended dilutions for Immunocytochemistry (1/1,000-1/5,000) Recommended dilutions for Western Blot (1/1,000-1/2,000)
Restrictions For Research Use only
Format Lyophilized
Stock 4 °C
Reference: Background King, Stinus, le Moal et al.: "Central administration of arginine vasotocin: effects on exploratory behavior in the rat." dans: Psychopharmacology, Vol. 76, Issue 1, pp. 40-3, 1982 (PubMed).

King, Geffard, Chavreau et al.: "Des-tyr-gamma-endorphin (DT gamma E) and pineal levels of melatonin and arginine vasopressin (AVP)." dans: European journal of pharmacology, Vol. 76, Issue 2-3, pp. 271-4, 1982 (PubMed).

N° du produit ABIN95359
531,14 €
Plus frais de livraison 40,00 € et TVA

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