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Strep Tag II anticorps (FITC)

IF, ICC, FACS Hôte: Souris Monoclonal 5A9F9 FITC
N° du produit ABIN962832
  • Antigène
    Strep Tag II
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 10
    • 4
    Souris
    Clonalité
    • 10
    • 4
    Monoclonal
    Conjugué
    • 8
    • 3
    • 2
    • 1
    FITC
    Application
    • 13
    • 8
    • 3
    • 2
    • 2
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Marque
    THE™
    Séquence
    NWSHPQFEK
    Specificité
    THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
    Attributs du produit
    The THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse is THETM NWSHPQFEK Tag Antibody conjugated with FITC. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.
    Purification
    Protein A affinity column
    Immunogène
    NWSHPQFEK peptide conjugated - KLH
    Clone
    5A9F9
    Isotype
    IgG1
  • Indications d'application
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    FACS: 1-3 µg for 1 x 10^6 cells
    ICC/IF: 1-3 µg/mL
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
    Buffer
    PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing cycles.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Antigène
    Strep Tag II
    Classe de substances
    Tag
    Sujet
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. Strep II tag (NWSHPQFEK) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
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