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V5 Epitope Tag anticorps (HRP)

WB, ELISA Hôte: Souris Monoclonal 4C12E11 HRP
N° du produit ABIN962844
  • Antigène Tous les produits V5 Epitope Tag
    V5 Epitope Tag
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 60
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    Souris
    Clonalité
    • 57
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    Monoclonal
    Conjugué
    • 66
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    Cet anticorp V5 Epitope Tag est conjugé à/à la HRP
    Application
    • 100
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    Western Blotting (WB), ELISA
    Marque
    THE™
    Séquence
    GKPIPNPLLG LDST
    Specificité
    THETM V5 Tag Antibody [HRP], mAb, Mouse recognizes C-terminal tagged fusion proteins, and it slightly recognizes N-terminal V5 tagged fusion proteins.
    Attributs du produit
    The THETM V5 Tag Antibody [HRP], mAb, Mouse is THETM V5 Tag Antibody, mAb, Mouse conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
    Purification
    Protein A affinity column
    Immunogène
    V5 tag peptide conjugated - KLH
    Clone
    4C12E11
    Isotype
    IgG2a
  • Indications d'application
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.1 µg/mL.Western blot: 0.2-1 µg/mL.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
    Buffer
    PBS, pH 7.4, containing 10 mg/mL BSA and 0.01 % Thimerosal.
    Agent conservateur
    Thimerosal (Merthiolate)
    Précaution d'utilisation
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing cycles.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Antigène
    V5 Epitope Tag
    Autre désignation
    V5 Tag (V5 Epitope Tag Produits)
    Classe de substances
    Tag
    Sujet
    V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
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