p21 anticorps
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- Antigène Voir toutes p21 (CDKN1A) Anticorps
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp p21 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
- Specificité
- This antibody reacts with human and other mammalian p21 protein. p21 is an intracellular protein of 21kD size. It is also known as the wild type p53 activated fragment 1 or WAF1. p21 inhibits cyclin-dependent kinases (Cdk's) and also proliferating cell nuclear antigens or PCNA's).
- Purification
- Concentrated.
- Immunogène
- Human p21 protein.
- Clone
- B459
- Isotype
- IgG1
- Top Product
- Discover our top product CDKN1A Anticorps primaire
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- Indications d'application
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Immunohistochemistry: 1:50-1:100 on tissue sections.
Western blotting: 1:100-1:300.
ELISA: 1:500-1:3000.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffinembedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory. - Commentaires
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Cellular Localization: Nuclear.
Recommended Positive Control: Breast, Skin, or Colon Carcinoma, Fibroadenoma. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.3 mg/ml.
- Buffer
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." dans: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, (1995) (PubMed).
: "The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition." dans: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17060-3, (1995) (PubMed).
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Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." dans: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, (1995) (PubMed).
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- Antigène
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- Autre désignation
- WAF 1/p21 Protein (CDKN1A Produits)
- Synonymes
- anticorps CCND1, anticorps CDKN1A, anticorps CAP20, anticorps CDKN1, anticorps CIP1, anticorps MDA-6, anticorps P21, anticorps SDI1, anticorps WAF1, anticorps p21CIP1, anticorps CDKI, anticorps Cdkn1, anticorps Waf1, anticorps mda6, anticorps p21Cip1, anticorps p21WAF, anticorps Cip1, anticorps UV96, anticorps p21, anticorps cip1, anticorps p16Xic2, anticorps sdi1, anticorps waf1, anticorps cap20, anticorps cdkn1, anticorps mda-6, anticorps p21cip1, anticorps si:ch1073-48m5.2, anticorps KRAS2, anticorps p21ras, anticorps cyclin D1, anticorps cyclin dependent kinase inhibitor 1A, anticorps cyclin-dependent kinase inhibitor 1A (P21), anticorps cyclin-dependent kinase inhibitor 1A, anticorps cyclin-dependent kinase inhibitor 1A L homeolog, anticorps cyclin-dependent kinase inhibitor 1, anticorps KRAS proto-oncogene, GTPase, anticorps CCND1, anticorps CDKN1A, anticorps Cdkn1a, anticorps cdkn1a.L, anticorps cki1, anticorps cdkn1a, anticorps KRAS
- Poids moléculaire
- 21 kDa
- Pathways
- Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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