PRKDC anticorps

N° du produit ABIN966893

Détail du produit anti-PRKDC anticorps

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKDC est non-conjugé

Application: Immunohistochemistry (IHC)

Immunogène: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to middle residues of human PRKDC(DNA-dependent protein kinase catalytic subunit)

Information d'application

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-PRKDC (ABIN966893)

Ladenburger, Fackelmayer, Hameister, Knippers: "MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13." dans: Cytogenetics and cell genetics, Vol. 77, Issue 3-4, pp. 268-70, (1997) (PubMed).

Sipley, Menninger, Hartley, Ward, Jackson, Anderson: "Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7515-9, (1995) (PubMed).

Poltoratsky, Shi, York, Lieber, Carter: "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4529-33, (1995) (PubMed).

Liu, Ma, Yueh, Lees-Miller, Anderson, Ng: "The carboxyl-terminal transactivation domain of human serum response factor contains DNA-activated protein kinase phosphorylation sites." dans: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21147-54, (1993) (PubMed).

Iijima, Teraoka, Date, Tsukada: "DNA-activated protein kinase in Raji Burkitt's lymphoma cells. Phosphorylation of c-Myc oncoprotein." dans: European journal of biochemistry / FEBS, Vol. 206, Issue 2, pp. 595-603, (1992) (PubMed).

Carter, Vancurová, Sun, Lou, DeLeon: "A DNA-activated protein kinase from HeLa cell nuclei." dans: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6460-71, (1991) (PubMed).

Lees-Miller, Anderson: "The human double-stranded DNA-activated protein kinase phosphorylates the 90-kDa heat-shock protein, hsp90 alpha at two NH2-terminal threonine residues." dans: The Journal of biological chemistry, Vol. 264, Issue 29, pp. 17275-80, (1989) (PubMed).

Détails sur PRKDC

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Autre désignation: PRKDC (PRKDC Produits)

Synonymes: anticorps DNA-PKcs, anticorps DNAPK, anticorps DNPK1, anticorps HYRC, anticorps HYRC1, anticorps XRCC7, anticorps p350, anticorps DNA-PKCS, anticorps dna-pkcs, anticorps dnapk, anticorps dnpk1, anticorps hyrc, anticorps hyrc1, anticorps prkdc-A, anticorps xrcc7, anticorps PRKDC, anticorps AI326420, anticorps AU019811, anticorps DNAPDcs, anticorps DOXNPH, anticorps dxnph, anticorps p460, anticorps scid, anticorps slip, anticorps protein kinase, DNA-activated, catalytic polypeptide, anticorps protein kinase, DNA-activated, catalytic polypeptide L homeolog, anticorps DNA-dependent protein kinase catalytic subunit, anticorps protein kinase, DNA activated, catalytic polypeptide, anticorps PRKDC, anticorps prkdc.L, anticorps LOC100633457, anticorps Prkdc

Sujet: PRKDC(DNA-dependent protein kinase catalytic subunit) is a Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. PRKDC promotes processing of hairpin DNA structures in V(D)Jrecombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. PRKDC is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. PRKDC found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. PRKDC also involved in modulation of transcription. PRKDC recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. PRKDC phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. PRKDC can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate TP53/p53 in the presence of supercoiled DNA is dependent on C1D.

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response