Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-residues of human XRCC4(X-ray repair cross-complementing protein 4)
XRCC4
Reactivité: Humain, Rat
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Restrictions
For Research Use only
Stock
4 °C
Nick McElhinny, Snowden, McCarville, Ramsden: "Ku recruits the XRCC4-ligase IV complex to DNA ends." dans: Molecular and cellular biology, Vol. 20, Issue 9, pp. 2996-3003, (2000) (PubMed).
Leber, Wise, Mizuta, Meek: "The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase." dans: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1794-801, (1998) (PubMed).
Grawunder, Wilm, Wu, Kulesza, Wilson, Mann, Lieber: "Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells." dans: Nature, Vol. 388, Issue 6641, pp. 492-5, (1997) (PubMed).
Antigène
XRCC4
(X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))
anticorps zgc:73312, anticorps MGC81443, anticorps homolog of human DNA ligase iv-binding protein XRCC4, anticorps 2310057B22Rik, anticorps AW413319, anticorps AW545101, anticorps X-ray repair cross complementing 4, anticorps X-ray repair complementing defective repair in Chinese hamster cells 4, anticorps X-ray repair complementing defective repair in Chinese hamster cells 4 L homeolog, anticorps DNA ligase IV-binding protein, anticorps XRCC4, anticorps xrcc4, anticorps xrcc4.L, anticorps Xrcc4
Sujet
XRCC4 (X-ray repair cross-complementing protein 4) is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. XRCC4 binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. XRCC4 is a homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. XRCC4 seems to interact directly with PRKDC but not with the Ku p70/86 dimer. It interacts with XLF/Cernunnos. Interacts with APTX and APLF. Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. It has no effect on ubiquitination.