BCL2L1 anticorps (AA 3-14)

N° du produit ABIN967301

Détail du produit anti-BCL2L1 anticorps

Antigène: BCL2L1 (BCL2-Like 1 (BCL2L1))

Épitope: AA 3-14

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BCL2L1 est non-conjugé

Application: Western Blotting (WB)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human, mouse Bcl-xL / Bcl-xS (aa. 3-14)

Clone: 2H12

Isotype: IgG2a

Information d'application

Indications d'application: In addition to Jurkat cell lysates, MOLT-4 (Human T-lymphoblasts, ATCC CRL-1582), K-562 (Human bone marrow myelogenous leukemia, ATCC CCL-243), Hut-78 (Human T lymphoma, ATCC TIB-161), human peripheral blood mononuclear cells (PBMC), rat thymocytes and mouse thymocytes have also been reported to be useful as positive controls.

Commentaires:

Related Products: ABIN968537

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-BCL2L1 (ABIN967301)

Hsu, Wolter, Youle: "Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3668-72, (1997) (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." dans: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, (1997) (PubMed).

Reed, Miyashita, Krajewski, Takayama, Aime-Sempe, Kitada, Sato, Wang, Harigai, Hanada, Krajewska, Kochel, Millan, Kobayashi: "Bcl-2 family proteins and the regulation of programmed cell death in leukemia and lymphoma." dans: Cancer treatment and research, Vol. 84, pp. 31-72, (1996) (PubMed).

Krajewski, Krajewska, Shabaik, Wang, Irie, Fong, Reed: "Immunohistochemical analysis of in vivo patterns of Bcl-X expression." dans: Cancer research, Vol. 54, Issue 21, pp. 5501-7, (1994) (PubMed).

Détails sur BCL2L1

Antigène: BCL2L1 (BCL2-Like 1 (BCL2L1))

Autre désignation: Bcl-x (BCL2L1 Produits)

Synonymes: anticorps BCL-XL/S, anticorps BCL2L, anticorps BCLX, anticorps BCLXL, anticorps BCLXS, anticorps Bcl-X, anticorps PPP1R52, anticorps bcl-xL, anticorps bcl-xS, anticorps Bcl(X)L, anticorps Bcl-XL, anticorps Bcl-Xs, anticorps Bcl2l, anticorps BclX, anticorps bcl-x, anticorps Bcl-xl, anticorps Bclx, anticorps bcl-X, anticorps Bcl-xL, anticorps BCL-XL, anticorps BCL2L1, anticorps bcl-XL, anticorps bcl-x(l), anticorps bcl-xl/s, anticorps bcl-xs, anticorps bcl2l, anticorps bclx, anticorps bclxl, anticorps xR11, anticorps bcl-xl, anticorps blp1, anticorps cb1021, anticorps zfBLP1, anticorps BCL2 like 1, anticorps BCL2-like 1, anticorps Bcl2-like 1, anticorps BCL2-like 1 L homeolog, anticorps bcl2-like 1, anticorps BCL2L1, anticorps Bcl2l1, anticorps bcl2l1, anticorps bcl2l1.L

Sujet: Members of the Bcl-2 family play a major role in regulating the response of cells to a wide variety of apoptotic signals. Some Bcl-2 family members like Bcl-2, block apoptosis, whereas others promote apoptosis and inhibit Bcl-2 activity. Bcl-x is a Bcl-2 homologue that has two isoforms, resulting from alternative splicing. Bcl-xL (long) is a 241 amino acid protein (25-29 kDa) that is 47% homologous to Bcl-2 on the amino acid level. Bcl-xS (short) is a 178 amino acid protein (~ 19.5 kDa) lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 protein family. Bcl-xL blocks cell death, whereas Bcl-xS inhibits Bcl-2 and promotes cell death. The 2H12 antibody has been reported to recognize human, mouse and rat Bcl-xL (long) protein. An N-terminal peptide (amino acids 3-14) common to human and mouse Bcl-xL and Bcl-xS (short) proteins was used as the immunogen. Thus the antibody is also predicted to recognize the Bcl-xS protein as the sequence used for the immunogen is common to both Bcl-xL and Bcl-xS proteins. Reports from development, however, have indicated that Bcl-xS has not been observable.

Poids moléculaire: 25-29 kDa

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling