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Caspase 8 anticorps (full length)

CASP8 Reactivité: Humain WB, IP Hôte: Souris Monoclonal 3-1 unconjugated
N° du produit ABIN967334
  • Antigène Voir toutes Caspase 8 (CASP8) Anticorps
    Caspase 8 (CASP8)
    Épitope
    • 16
    • 16
    • 10
    • 7
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    full length
    Reactivité
    • 138
    • 70
    • 63
    • 23
    • 16
    • 16
    • 10
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 130
    • 25
    • 6
    • 2
    Souris
    Clonalité
    • 127
    • 35
    Monoclonal
    Conjugué
    • 98
    • 16
    • 11
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp Caspase 8 est non-conjugé
    Application
    • 143
    • 50
    • 44
    • 39
    • 27
    • 26
    • 25
    • 21
    • 19
    • 18
    • 8
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human Caspase-8 full-length recombinant protein
    Clone
    3-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CASP8 Anticorps primaire
  • Indications d'application
    Applications include western blot analysis ( 0.125 - 0.5 µg /ml) and immunoprecipitation (4 µg /200µg cell lysate ). Jurkat T cells ( ATCC CRL-1573) are suggested as a positive control. Antibodies-online offers several caspase-8 antibodies. A Jurkat model cell system was used to evaluate these antibodies, these results are summarized in the following table. However, actual bands observed could vary according to the cell model system or treatment used.
    Commentaires

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Boesen-de Cock, Tepper, de Vries, van Blitterswijk, Borst: "Common regulation of apoptosis signaling induced by CD95 and the DNA-damaging stimuli etoposide and gamma-radiation downstream from caspase-8 activation." dans: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14255-61, (1999) (PubMed).

    Thome, Martinon, Hofmann, Rubio, Steiner, Schneider, Mattmann, Tschopp: "Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase." dans: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 9962-8, (1999) (PubMed).

    Cock, Tepper, de Vries, van Blitterswijk, Borst: "CD95 (Fas/APO-1) induces ceramide formation and apoptosis in the absence of a functional acid sphingomyelinase." dans: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7560-5, (1998) (PubMed).

  • Antigène
    Caspase 8 (CASP8)
    Autre désignation
    Caspase-8 (CASP8 Produits)
    Synonymes
    anticorps casp8-A, anticorps xCaspase-8, anticorps casp8, anticorps CASP8, anticorps CASP-8, anticorps FLICE, anticorps MACH, anticorps Mch5, anticorps ALPS2B, anticorps CAP4, anticorps Casp-8, anticorps MCH5, anticorps caspase-8, anticorps zgc:92075, anticorps caspase 8 L homeolog, anticorps death related ced-3/Nedd2-like protein, anticorps caspase 8, anticorps caspase-8, anticorps caspase-8-like, anticorps caspase 8, apoptosis-related cysteine peptidase, anticorps casp8.L, anticorps LOC661095, anticorps CASP8, anticorps LOC5563818, anticorps casp8, anticorps LOC100222284, anticorps Casp8
    Sujet
    Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. The N-terminal region of caspase-8 contains an amino acid sequence, termed the death domain, that facilitates caspase-8-FADD direct interaction. FADD therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. Viral proteins (v-FLIPS) which inhibit recruitment and activation of caspase-8 have been isolated. Caspase-8 is produced as a proenzyme (55/50 kDa doublet) which upon receptor aggregation is proteolytically cleaved into smaller subunits of 40/36 (doublet), and 23 kDa. Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g., MCF-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, CrmA. The antibody recognizes both the proform of human caspase-8 (55/50 kDa doublet) as well as the cleaved forms which migrate at 40/36 (doublet) and 23 kDa in SDS/PAGE. It also immunoprecipitates the full-length human caspase-8. Full-length recombinant human caspase-8 protein was used as immunogen.
    Synonyms: FLICE, MACH-1, Mch5
    Poids moléculaire
    55/50 kDa, 40/36 kDa, 23 kDa
    Pathways
    Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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