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SYK anticorps (AA 314-339)

SYK Reactivité: Humain WB, FACS Hôte: Souris Monoclonal 4D10 unconjugated
N° du produit ABIN967352
  • Antigène Voir toutes SYK Anticorps
    SYK (Spleen tyrosine Kinase (SYK))
    Épitope
    • 29
    • 22
    • 20
    • 16
    • 10
    • 10
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 314-339
    Reactivité
    • 175
    • 88
    • 53
    • 11
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    Humain
    Hôte
    • 158
    • 22
    • 3
    Souris
    Clonalité
    • 161
    • 21
    Monoclonal
    Conjugué
    • 106
    • 12
    • 10
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp SYK est non-conjugé
    Application
    • 148
    • 84
    • 46
    • 26
    • 23
    • 19
    • 14
    • 14
    • 13
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human Syk peptide aa. 314-339
    Clone
    4D10
    Isotype
    IgG2a
    Top Product
    Discover our top product SYK Anticorps primaire
  • Indications d'application
    Western Blot: EB1 control lysate [50 µg (1 µg/ml)] is provided as a positive control (store lysate at -20°C).
    Additional application: The FITC-conjugated format of clone 4D10 is useful in immunofluorescent staining and flow cytometric analysis.
    Commentaires

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    SDS-PAGE buffer (62 mM Tris pH 6.8, 2 % SDS, 0.9 % b-mercaptoethanol, 0.003 % bromophenol blue, 5 % glycerol)
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20°C.
  • Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." dans: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, (2001) (PubMed).

    Turner, Schweighoffer, Colucci, Di Santo, Tybulewicz: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." dans: Immunology today, Vol. 21, Issue 3, pp. 148-54, (2000) (PubMed).

    Chu, van Oers, Malissen, Harris, Elder, Weiss: "Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2610-20, (1999) (PubMed).

    Costello, Turner, Walters, Cunningham, Bauer, Downward, Tybulewicz: "Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells." dans: Oncogene, Vol. 13, Issue 12, pp. 2595-605, (1997) (PubMed).

  • Antigène
    SYK (Spleen tyrosine Kinase (SYK))
    Autre désignation
    Syk (SYK Produits)
    Synonymes
    anticorps p72-Syk, anticorps Sykb, anticorps p72syk, anticorps spleen associated tyrosine kinase, anticorps spleen tyrosine kinase, anticorps syk, anticorps SYK, anticorps Syk
    Sujet
    Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igalpha, TCRzeta, CD3epsilon, FcepsilonRIbeta, and FceRIgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cgamma1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa, Accession number P43405).
    Pathways
    Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling
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