CD3 epsilon anticorps

N° du produit ABIN967370

Détail du produit anti-CD3 epsilon anticorps

Antigène: CD3 epsilon (CD3E)

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Cytotoxicity Test (CyTox)

Marque: BD Pharmingen™

Attributs du produit: 1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: H-2Kb specific cytotoxic T lymphocyte clone BM10-37

Clone: 145-2C11

Isotype: IgG1 kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-CD3 epsilon (ABIN967370)

Martin, Lenardo: "Morphological, biochemical, and flow cytometric assays of apoptosis." dans: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Vol. Chapter 14, pp. Unit 14.13, (2008) (PubMed).

Kruisbeek, Shevach, Thornton: "Proliferative assays for T cell function." dans: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 3, pp. Unit 3.12, (2008) (PubMed).

Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." dans: International immunology, Vol. 6, Issue 7, pp. 995-1001, (1994) (PubMed).

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e (CD3E Produits)

Synonymes: anticorps cd3e, anticorps CD3E, anticorps t3e, anticorps tcre, anticorps AI504783, anticorps CD3, anticorps CD3epsilon, anticorps T3e, anticorps T3E, anticorps TCRE, anticorps CD3e molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps cd3e, anticorps CD3E, anticorps Cd3e

Sujet: The 145-2C11 antibody reacts with the 25-kDa epsilon chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3epsilon and anti-alphabeta TCR mAbs to detect the T-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis.
Synonyms: CD3epsilon chain

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway