NTAN1 anticorps

N° du produit ABIN967387

Détail du produit anti-NTAN1 anticorps

Antigène: NTAN1 (N-terminal Asparagine Amidase (NTAN1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp NTAN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Marque: BD Pharmingen™

Specificité: Carbohydrate Epitope

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Collagenase-dispersed BALB/c lymph node stroma

Clone: MECA-79

Isotype: IgM kappa

Information d'application

Indications d'application: This antibody has been tested by immunohistochemical staining (IHC) of citrate-pretreated formalin-fixed paraffin-embedded sections (5 - 20 µg/ml) to assure specificity and reactivity. Other reported applications include IHC of acetone-fixed frozen sections, immunoprecipitation, western blot analysis, and in vitro and in vivo adhesion blocking.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-NTAN1 (ABIN967387)

Jentho, Bodden, Schulz, Jung, Seidel, Schmeck, Bertrams: "microRNA-125a-3p is regulated by MyD88 in Legionella pneumophila infection and targets NTAN1." dans: PLoS ONE, Vol. 12, Issue 4, pp. e0176204, (2017) (PubMed).

Binns, Whyte, Licence, Harrison, Tsang, Haskard, Robinson: "The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 4094-9, (1996) (PubMed).

Diacovo, Puri, Warnock, Springer, von Andrian: "Platelet-mediated lymphocyte delivery to high endothelial venules." dans: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 252-5, (1996) (PubMed).

Malý, Thall, Petryniak, Rogers, Smith, Marks, Kelly, Gersten, Cheng, Saunders, Camper, Camphausen, Sullivan, Isogai, Hindsgaul, von Andrian, Lowe: "The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis." dans: Cell, Vol. 86, Issue 4, pp. 643-53, (1996) (PubMed).

Puri, Finger, Gaudernack, Springer: "Sialomucin CD34 is the major L-selectin ligand in human tonsil high endothelial venules." dans: The Journal of cell biology, Vol. 131, Issue 1, pp. 261-70, (1995) (PubMed).

Faveeuw, Gagnerault, Lepault: "Expression of homing and adhesion molecules in infiltrated islets of Langerhans and salivary glands of nonobese diabetic mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 12, pp. 5969-78, (1994) (PubMed).

Hemmerich, Butcher, Rosen: "Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody." dans: The Journal of experimental medicine, Vol. 180, Issue 6, pp. 2219-26, (1994) (PubMed).

Michie, Streeter, Bolt, Butcher, Picker: "The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing." dans: The American journal of pathology, Vol. 143, Issue 6, pp. 1688-98, (1994) (PubMed).

Berg, Robinson, Warnock, Butcher: "The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor." dans: The Journal of cell biology, Vol. 114, Issue 2, pp. 343-9, (1991) (PubMed).

Streeter, Rouse, Butcher: "Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes." dans: The Journal of cell biology, Vol. 107, Issue 5, pp. 1853-62, (1988) (PubMed).

Détails sur NTAN1

Antigène: NTAN1 (N-terminal Asparagine Amidase (NTAN1))

Autre désignation: PNAd (NTAN1 Produits)

Synonymes: anticorps PNAA, anticorps PNAD, anticorps N-terminal Asn amidase, anticorps N-terminal asparagine amidase, anticorps Ntan1, anticorps NTAN1

Sujet: The MECA-79 antibody reacts with sulfate-dependent carbohydrate epitopes of peripheral lymph node addressin (PNAd). The MECA-79-reactive antigen is closely associated with the carbohydrate ligands for L-selectin (eg, CD34, GlyCAM-1, MAdCAM-1), which are expressed on high endothelial venules (HEV) in lymphoid tissues and at sites of chronic inflammation. Cross-reactivity with human, sheep, cow, primate, and pig tissues has been observed. MECA-79 antibody inhibits L-selectin-dependent lymphocyte and platelet homing to lymph nodes in vivo, and in vitro adhesion to lymphoid tissue HEV and immobilized PNAd.
Synonyms: CD62L Ligand