TCF3 anticorps
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- Antigène Voir toutes TCF3 Anticorps
- TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))
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Reactivité
- Humain, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp TCF3 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Marque
- BD Pharmingen™
- Réactivité croisée
- Souris
- Attributs du produit
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Recombinant Human E47
- Clone
- G127-32
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product TCF3 Anticorps primaire
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- Indications d'application
- Applications include immunoprecipitation, western blot analysis (1-2 µg/ml) and electropheretic mobility shift assays (EMSA). Please refer to reference Bain G, et al, 1993 for suggested methods.
- Commentaires
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Related Products: ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C.
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Both E12 and E47 allow commitment to the B cell lineage." dans: Immunity, Vol. 6, Issue 2, pp. 145-54, (1997) (PubMed).
: "E2A and E2-2 are subunits of B-cell-specific E2-box DNA-binding proteins." dans: Molecular and cellular biology, Vol. 13, Issue 6, pp. 3522-9, (1993) (PubMed).
: "A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins." dans: Cell, Vol. 56, Issue 5, pp. 777-83, (1989) (PubMed).
: "Protein-binding sites in Ig gene enhancers determine transcriptional activity and inducibility." dans: Science (New York, N.Y.), Vol. 236, Issue 4808, pp. 1573-7, (1987) (PubMed).
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Both E12 and E47 allow commitment to the B cell lineage." dans: Immunity, Vol. 6, Issue 2, pp. 145-54, (1997) (PubMed).
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- Antigène
- TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))
- Autre désignation
- E2A (TCF3 Produits)
- Synonymes
- anticorps E2A, anticorps E47, anticorps ITF1, anticorps TCF-3, anticorps VDIR, anticorps bHLHb21, anticorps tcf3a, anticorps zgc:113045, anticorps A1, anticorps AA408400, anticorps ALF2, anticorps AW209082, anticorps E12, anticorps E12/E47, anticorps ME2, anticorps Pan1, anticorps Pan2, anticorps Tcfe2a, anticorps bhlhb21, anticorps e2a, anticorps itf1, anticorps tcfe2a, anticorps vdir, anticorps xtcf-3, anticorps xtcf3, anticorps G12, anticorps TCF7L1, anticorps XE3, anticorps transcription factor 3, anticorps transcription factor 3b, anticorps transcription factor 3 L homeolog, anticorps transcription factor E2a, anticorps TCF3, anticorps tcf3b, anticorps Tcf3, anticorps tcf3.L, anticorps tcfe2a
- Sujet
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B lymphoid cells go through many intermediate steps before becoming plasma cells which produce and secrete immunoglobulins (Ig). The earliest stage of B-cell differentiation is represented by pro-B lymphocytes that contain both the Ig heavy- and light-chain genes in the transcriptionally inactive germ line configuration. As pro-B lymphocytes differentiate into pre-B cells, Ig heavy-chain genes rearrange and are transcribed and translated. However, the Ig light-chain genes are not transcribed before the pre-B cells differentiate into mature Blymphocytes. The developmental regulation of the Ig gene expression is dependent on various sequences in the Ig enhancer region. One class of such regulatory sequence elements comprises the E-boxes which share the NNCANNTGNN consensus sequence. The E2 boxes are particularly interesting because they are present in muscle and pancreas-specific enhancers. A family of proteins binds to the E2 box. These proteins share a common amino acid sequence motif that is proposed to form two amphipathic helices interrupted by a loop, designated the helix-loop-helix (HLH) motif. The HLH motif mediates homo- as well as heterodimerization with other HLH proteins. Most HLH proteins possess a basic region located at the N terminal of the HLH region which is responsible for DNA binding. Two E2 box binding proteins have been described (E12 and E47) that arise as alternatively spliced form of the E2A gene. E12 and E47 are identical except in the HLH region. These two proteins have been directly implicated in the regulation of B-cell, muscle and pancreas-specific gene expression. Futhermore, studies have shown that relative levels of E47 are important for regulating the lineage of T cells in the thymus. For example, mice deficient in E47 demonstrate a change in the ratio of CD4/CD8 cells as well as a greater percentage of cells that stain for the mature CD4 marker, implicating E47 in selection of T cells. Clone G127-32 recognizes human E47. It cross-reacts with mouse E47. G127-32 does not recognize E12, the alternately spliced product of the E2A gene or E2-2, a closely reltated protein. Recombinant E47 was used as immunogen.
Synonyms: E47(E2A), Immunoglobulin E2-Box Binding Protein - Poids moléculaire
- 75 kDa
- Pathways
- Signalisation WNT, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response
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