Retinoblastoma 1 anticorps (underphosphorylated)

N° du produit ABIN967418

Détail du produit anti-Retinoblastoma 1 anticorps

Antigène: Retinoblastoma 1 (RB1)

Épitope: AA 514-610, underphosphorylated

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Retinoblastoma 1 est non-conjugé

Application: Immunoprecipitation (IP)

Marque: BD Pharmingen™

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant full-length human Rb

Clone: G99-549

Isotype: IgG1

Information d'application

Indications d'application: Applications include immunoprecipitation at 1-2 µg/one million cells. Rb migrates as multiple, closely-spaced bands between approximately 110-116 kDa when sized on denaturing polyacrylamide gels (i.e. by SDS/PAGE). The multiple bands represent different Rb phosphorylation states, the higher molecular weight bands are more highly phosphorylated than the lower molecular weight bands. The level of phosphorylation is cell cycle dependent, and may also be cell type dependent (not all forms are seen in all cell types that express Rb). Gel conditions influence the actual number of bands observed. In cases where optimal band separation is desired, use a 7 to 10% non-gradient long (at least 12 inches) gel. MOLT-4 human leukemia cells (ATCC CRL-1582) are suggested as a positive control.

Commentaires:

Related Products: ABIN967389, ABIN967405,

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Retinoblastoma 1 (ABIN967418)

Riley, Lee, Lee: "The retinoblastoma protein: more than a tumor suppressor." dans: Annual review of cell biology, Vol. 10, pp. 1-29, (1995) (PubMed).

Cordon-Cardo, Richon: "Expression of the retinoblastoma protein is regulated in normal human tissues." dans: The American journal of pathology, Vol. 144, Issue 3, pp. 500-10, (1994) (PubMed).

Dunaief, Strober, Guha, Khavari, Alin, Luban, Begemann, Crabtree, Goff: "The retinoblastoma protein and BRG1 form a complex and cooperate to induce cell cycle arrest." dans: Cell, Vol. 79, Issue 1, pp. 119-30, (1994) (PubMed).

Hollingsworth, Chen, Lee: "Integration of cell cycle control with transcriptional regulation by the retinoblastoma protein." dans: Current opinion in cell biology, Vol. 5, Issue 2, pp. 194-200, (1993) (PubMed).

Livingston: "Functional analysis of the retinoblastoma gene product and of RB-SV40 T antigen complexes." dans: Cancer surveys, Vol. 12, Issue 6, pp. 153-60, (1992) (PubMed).

Weinberg: "Tumor suppressor genes." dans: Science (New York, N.Y.), Vol. 254, Issue 5035, pp. 1138-46, (1991) (PubMed).

Détails sur Retinoblastoma 1

Antigène: Retinoblastoma 1 (RB1)

Autre désignation: Rb (RB1 Produits)

Synonymes: anticorps OSRC, anticorps RB, anticorps p105-Rb, anticorps pRb, anticorps pp110, anticorps Rb, anticorps Rb-1, anticorps P105-RB, anticorps PP110, anticorps RB11, anticorps zgc:154147, anticorps RB transcriptional corepressor 1, anticorps RB transcriptional corepressor like 1, anticorps retinoblastoma 1, anticorps RB1, anticorps RBL1, anticorps Rb1, anticorps rb1

Sujet: The retinoblastoma gene encodes a nuclear phosphoprotein (Rb or p110Rb) which is expressed in most normal cells of vertebrates and acts as a tumor suppressor gene product. An underphosphorylated form of Rb is mainly found in resting or fully differentiated cells, whereas the hyperphosphorylated form is present in proliferating cells. Only the underphosphorylated form of Rb binds specifically to viral oncogenes such as SV40 large T, adenoviral EIA and HPV-E7. This interaction may partially contribute to the transforming activity of these viral oncoproteins. Rb also interacts with several cyclins including A, D, and E as well as the transcriptional activator E2F. The importance of these interactions for the biological function of Rb is still being elucidated. G99-549 recognizes only the fast migrating, underphosphorylated form of Rb protein. G99-549 cross-reacts with mouse underphosphorylated Rb. The epitope has been mapped to amino acids 514-610 (box A) of human Rb. Recombinant full-length human Rb was produced in the baculovirus expression vector system (BEVS), purified and used as immunogen.
Synonyms: RB (a.a. 514-610)

Poids moléculaire: 105 kDa

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy