Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

FLI1 anticorps

FLI1 Reactivité: Humain WB, IP Hôte: Souris Monoclonal G146-222 unconjugated
N° du produit ABIN967450
  • Antigène Voir toutes FLI1 Anticorps
    FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
    Reactivité
    • 73
    • 27
    • 19
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    Humain
    Hôte
    • 50
    • 21
    • 3
    Souris
    Clonalité
    • 50
    • 24
    Monoclonal
    Conjugué
    • 53
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp FLI1 est non-conjugé
    Application
    • 62
    • 28
    • 25
    • 17
    • 15
    • 11
    • 7
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Fli-1 ets Domain Fusion Protein
    Clone
    G146-222
    Isotype
    IgG2b
    Top Product
    Discover our top product FLI1 Anticorps primaire
  • Indications d'application
    G146-222 has been shown to recognize in vitro translated, recombinant bacterially expressed and endogenous B cell Fli-1. In gel shift assays, this antibody disrupts complexes of Fli-1 and DNA probe, however, this application is not tested.
    Commentaires

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Bailly, Bosselut, Zucman, Cormier, Delattre, Roussel, Thomas, Ghysdael: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." dans: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, (1994) (PubMed).

    May, Lessnick, Braun, Klemsz, Lewis, Lunsford, Hromas, Denny: "The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1." dans: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7393-8, (1994) (PubMed).

  • Antigène
    FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
    Autre désignation
    Fli-1 (FLI1 Produits)
    Synonymes
    anticorps EWSR2, anticorps SIC-1, anticorps FLI1, anticorps fli, anticorps Xfli, anticorps fli1, anticorps Xfli1, anticorps fli-1, anticorps Xfli-1, anticorps MGC121593, anticorps Fli-1, anticorps Sic1, anticorps cb855, anticorps wu:fc45b11, anticorps xlfli, anticorps Fli-1 proto-oncogene, ETS transcription factor, anticorps Fli-1 proto-oncogene, ETS transcription factor S homeolog, anticorps Friend leukemia integration 1, anticorps Fli-1 proto-oncogene, ETS transcription factor a, anticorps Fli-1 proto-oncogene, ETS transcription factor L homeolog, anticorps FLI1, anticorps Fli1, anticorps fli1.S, anticorps fli1, anticorps fli1a, anticorps fli1.L
    Sujet
    Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through an 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma (EWS) through a t(11,22)(q24,q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli- 1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to transactivate the promoter for c-myc. G146-222 recognizes human Fli-1. The antibody was raised against a bacterially expressed Fli-1 ets domain fusion protein.
    Poids moléculaire
    50 kDa
Vous êtes ici:
Support technique