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SERPINB5 anticorps

SERPINB5 Reactivité: Humain WB Hôte: Souris Monoclonal G167-70 unconjugated
N° du produit ABIN967456
  • Antigène Voir toutes SERPINB5 Anticorps
    SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))
    Reactivité
    • 75
    • 38
    • 32
    • 17
    • 4
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 67
    • 7
    • 1
    Souris
    Clonalité
    • 67
    • 8
    Monoclonal
    Conjugué
    • 41
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp SERPINB5 est non-conjugé
    Application
    • 68
    • 33
    • 30
    • 29
    • 15
    • 14
    • 13
    • 13
    • 8
    • 7
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Recombinant Human Maspin
    Clone
    G167-70
    Isotype
    IgG2b
    Top Product
    Discover our top product SERPINB5 Anticorps primaire
  • Indications d'application
    Applications include western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL 1555) are recommended as a positive control for this application.
    Commentaires

    Related Products: ABIN967389, ABIN968533

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Sheng, Pemberton, Sager: "Production, purification, and characterization of recombinant maspin proteins." dans: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30988-93, (1995) (PubMed).

    Hopkins, Whisstock: "Function of maspin." dans: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1893-4, (1994) (PubMed).

    Zou, Anisowicz, Hendrix, Thor, Neveu, Sheng, Rafidi, Seftor, Sager: "Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells." dans: Science (New York, N.Y.), Vol. 263, Issue 5146, pp. 526-9, (1994) (PubMed).

  • Antigène
    SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))
    Autre désignation
    Maspin (SERPINB5 Produits)
    Synonymes
    anticorps 1110036M19Rik, anticorps AI462524, anticorps AI646751, anticorps Maspin, anticorps PI-5, anticorps Spi7, anticorps ovalbumin, anticorps PI5, anticorps maspin, anticorps Pi5, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 5, anticorps serpin family B member 5, anticorps serpin peptidase inhibitor, clade B (ovalbumin), member 5 S homeolog, anticorps Serpinb5, anticorps SERPINB5, anticorps serpinb5.S
    Sujet
    Maspin is a 42 kDa protein that may play a role in human breast cancer by functioning as a tumor suppressor. Maspin is expressed in normal mammary epithelial cells, but there is a loss of expression in invasive breast cancer including lymph node and distant metastases. Further, maspin is not expressed in most mammary carcinoma cell lines. Mammary carcinoma cells transfected with recombinant maspin have a reduced ability to migrate through reconstituted basement membrane and lose their ability to form tumors in nude mice, providing support for maspin as a tumor suppressor protein. Maspin has homology to members of the serpin family of protease inhibitors, some of which have been implicated in the regulation of invasion and metastasis. Yet protease inhibitory activity has not been detected using recombinant maspin. It has been suggested that maspin, unlike most serpins, may be a ligand-binding serpin rather than a protease inhibitor. However, the question of whether or not maspin has protease inhibitor activity remains to be determined. G167-70 is specific for human maspin. It detects maspin as a 42 kD protein in western blots of normal human mammary epithelial cells. Many mammary tumor cell lines including MDA-MB-231 (ATCC HTB 26) and MDA-MB-435S (ATCC HTB 129) lack maspin expression and are suitable as negative controls. Purified recombinant human maspin from a yeast expression system was used as immunogen.
    Poids moléculaire
    42 kDa
    Pathways
    Signalisation p53
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