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IL-4 anticorps

IL4 Reactivité: Humain WB, Inhibition Hôte: Rat Monoclonal MP4-25D2 unconjugated
N° du produit ABIN967463
  • Antigène Voir toutes IL-4 (IL4) Anticorps
    IL-4 (IL4) (Interleukin 4 (IL4))
    Reactivité
    • 146
    • 67
    • 24
    • 14
    • 11
    • 10
    • 10
    • 8
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Humain
    Hôte
    • 110
    • 74
    • 63
    • 7
    • 3
    • 2
    Rat
    Clonalité
    • 149
    • 107
    • 1
    Monoclonal
    Conjugué
    • 150
    • 33
    • 17
    • 14
    • 10
    • 8
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp IL-4 est non-conjugé
    Application
    • 123
    • 117
    • 73
    • 72
    • 25
    • 24
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    • 18
    • 13
    • 13
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    • 9
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    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Blocking Antibody (Inhibition)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Purified Recombinant Human IL-4
    Clone
    MP4-25D2
    Isotype
    IgG1
    Top Product
    Discover our top product IL4 Anticorps primaire
  • Indications d'application
    1. Blocking Control for Intracellular Staining:
    The unlabeled MP4-25D2 antibody can be used as a blocking control to demonstrate specificity of IL-4 staining by conjugated MP4-25D2 antibody. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled MP4-25D2 antibody (ABIN967463) for 20 minutes at 4°C, prior to staining with conjugated MP4-25D2 antibody. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

    2. Neutralization:
    The NA/LE™ MP4-25D2 antibody is useful for neutralization of human IL-4 bioactivity. A suitable NA/LE ™ rat IgG1 isotype control to match the MP4-25D2 antibody is the R3-34 antibody.

    3. WB: The purified MP4-25D2 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4° C.
  • Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

    Jung, Schauer, Rieger, Wagner, Einsle, Neumann, Heusser: "Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells." dans: European journal of immunology, Vol. 25, Issue 8, pp. 2413-6, (1995) (PubMed).

    Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." dans: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, (1993) (PubMed).

    Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." dans: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

    Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." dans: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, (1989) (PubMed).

  • Antigène
    IL-4 (IL4) (Interleukin 4 (IL4))
    Autre désignation
    IL-4 (IL4 Produits)
    Synonymes
    anticorps IL4, anticorps IL-4, anticorps BCGF-1, anticorps BCGF1, anticorps BSF-1, anticorps BSF1, anticorps Il-4, anticorps Il4e12, anticorps IL2, anticorps interleukin 4, anticorps IL4, anticorps Il4
    Sujet
    The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described. This antibody is routinely tested as a blocking control for intracellular staining.
    Pathways
    Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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