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GM-CSF anticorps

CSF2 Reactivité: Humain WB, IP, Inhibition Hôte: Rat Monoclonal BVD2-21C11 unconjugated
N° du produit ABIN967465
  • Antigène Voir toutes GM-CSF (CSF2) Anticorps
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Reactivité
    • 115
    • 47
    • 20
    • 10
    • 10
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Humain
    Hôte
    • 90
    • 44
    • 29
    • 4
    • 1
    Rat
    Clonalité
    • 96
    • 72
    Monoclonal
    Conjugué
    • 87
    • 25
    • 8
    • 8
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GM-CSF est non-conjugé
    Application
    • 123
    • 72
    • 40
    • 33
    • 31
    • 26
    • 20
    • 16
    • 15
    • 11
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Blocking Antibody (Inhibition)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
    5. Cy is a trademark of Amersham Biosciences Limited.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Recombinant human GM-CSF
    Clone
    BVD2-21C11
    Isotype
    IgG2a
    Top Product
    Discover our top product CSF2 Anticorps primaire
  • Indications d'application
    Blocking Control for Intracellular Staining:
    The purified BVD2-21C11 antibody (ABIN967465) can be used as a blocking control to demonstrate specificity of human GM-CSF staining by the PE-BVD2-21C11. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1 - 10 µg of unlabeled BVD2-21C11 antibody (ABIN967465) for 20 minutes at 4°C, prior to staining with PE-BVD2-21C11 antibody (eg, 0.1 - 0.5 µg mAb/ 1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

    ELISA Detection:
    The biotinylated BVD2-21C11 antibody, is useful as a detection antibody for a sandwich ELISA for measuring human GM-CSF protein levels. Biotinylated BVD2-21C11 antibody can be paired with the purified BVD2-23B6 antibody as the capture antibody, with recombinant human GM-CSF as the standard. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." dans: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

    Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

    Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." dans: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

    Kita, Ohnishi, Okubo, Weiler, Abrams, Gleich: "Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils." dans: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 745-8, (1991) (PubMed).

    Bacchetta, de Waal Malefijt, Yssel, Abrams, de Vries, Spits, Roncarolo: "Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 902-8, (1990) (PubMed).

  • Antigène
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    Autre désignation
    GM-CSF (CSF2 Produits)
    Synonymes
    anticorps GMCSF, anticorps GM-CSF, anticorps CSF2, anticorps Csfgm, anticorps Gm-CSf, anticorps MGI-IGM, anticorps Gm-csf, anticorps Gmcsf, anticorps CSF, anticorps colony stimulating factor 2, anticorps colony stimulating factor 2 (granulocyte-macrophage), anticorps CSF2, anticorps Csf2
    Sujet
    The BVD2-21C11 monoclonal antibody specifically binds to human Granulocyte/Macrophage - Colony Stimulating Factor (GM-CSF). Human GM-CSF is encoded by the CSF2 gene and is also known as Colony Stimulating Factor 2. GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, stromal cells and other cell types including B lymphocytes, mast cells, eosinophils, and osteoblasts. GM-CSF stimulates the survival, proliferation and/or differentiation of various cell types including neutrophils, eosinophils, macrophages, dendritic cells, megakaryocytes, erythroid cells, endothelial cells and their precursors. The immunogen used to generate the BVD2-21C11 hybridoma was recombinant human GM-CSF. The BVD2-21C11 antibody has been reported to crossreact with GM-CSF from the rhesus monkey. BVD2-21C11 is a neutralizing antibody.
    Synonyms: CSF2, Colony stimulating factor 2 (granulocyte-macrophage), CSF, GMCSF
    Pathways
    Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin
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