ASCL1 anticorps

N° du produit ABIN967560

Détail du produit anti-ASCL1 anticorps

Antigène: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ASCL1 est non-conjugé

Application: Western Blotting (WB)

Marque: BD Pharmingen™

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat MASH1 full length recombinant protein

Clone: 24B72D11-1

Isotype: IgG1

Information d'application

Indications d'application: The 24B72D11.1 antibody is recommended for western blot analysis (0.5-2.0 µg/ml). Rat embryonic brain can be used as a positive control. Neuro2A mouse neuroblastoma cells (ATCC CCL-131) may also be used as a positive control for this application.

The 24B72D11.1 antibody is reported in the literature to work well for immunohistochemistry.

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-ASCL1 (ABIN967560)

Wang, Long, Flandin, Pla, Waclaw, Campbell, Rubenstein: "Loss of Gsx1 and Gsx2 function rescues distinct phenotypes in Dlx1/2 mutants." dans: The Journal of comparative neurology, Vol. 521, Issue 7, pp. 1561-84, (2013) (PubMed).

Krolewski, Packard, Schwob: "Global expression profiling of globose basal cells and neurogenic progression within the olfactory epithelium." dans: The Journal of comparative neurology, Vol. 521, Issue 4, pp. 833-59, (2013) (PubMed).

Sawamoto, Hirota, Alfaro-Cervello, Soriano-Navarro, He, Hayakawa-Yano, Yamada, Hikishima, Tabata, Iwanami, Nakajima, Toyama, Itoh, Alvarez-Buylla, Garcia-Verdugo, Okano: "Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain." dans: The Journal of comparative neurology, Vol. 519, Issue 4, pp. 690-713, (2011) (PubMed).

Guo, Packard, Krolewski, Harris, Manglapus, Schwob: "Expression of pax6 and sox2 in adult olfactory epithelium." dans: The Journal of comparative neurology, Vol. 518, Issue 21, pp. 4395-418, (2011) (PubMed).

Ivaniutsin, Chen, Mason, Price, Pratt: "Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex." dans: Neural development, Vol. 4, Issue 1, pp. 3, (2009) (PubMed).

Puverel, Nakatani, Parras, Soussi-Yanicostas: "Prokineticin receptor 2 expression identifies migrating neuroblasts and their subventricular zone transient-amplifying progenitors in adult mice." dans: The Journal of comparative neurology, Vol. 512, Issue 2, pp. 232-42, (2009) (PubMed).

Yu, Fotaki, Mason, Price: "Analysis of early ventral telencephalic defects in mice lacking functional Gli3 protein." dans: The Journal of comparative neurology, Vol. 512, Issue 5, pp. 613-27, (2009) (PubMed).

Ralston, Chiriboga, Nonaka: "MASH1: a useful marker in differentiating pulmonary small cell carcinoma from Merkel cell carcinoma." dans: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 11, pp. 1357-62, (2008) (PubMed).

Seki, Namba, Mochizuki, Onodera: "Clustering, migration, and neurite formation of neural precursor cells in the adult rat hippocampus." dans: The Journal of comparative neurology, Vol. 502, Issue 2, pp. 275-90, (2007) (PubMed).

Hu, Ippolito, Garabedian, Humphrey, Gordon: "Molecular characterization of a metastatic neuroendocrine cell cancer arising in the prostates of transgenic mice." dans: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44462-74, (2002) (PubMed).

Sommer, Shah, Rao, Anderson: "The cellular function of MASH1 in autonomic neurogenesis." dans: Neuron, Vol. 15, Issue 6, pp. 1245-58, (1996) (PubMed).

Johnson, Birren, Saito, Anderson: "DNA binding and transcriptional regulatory activity of mammalian achaete-scute homologous (MASH) proteins revealed by interaction with a muscle-specific enhancer." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 8, pp. 3596-600, (1992) (PubMed).

Lo, Johnson, Wuenschell, Saito, Anderson: "Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells." dans: Genes & development, Vol. 5, Issue 9, pp. 1524-37, (1991) (PubMed).

Détails sur ASCL1

Antigène: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Autre désignation: MASH1 (ASCL1 Produits)

Synonymes: anticorps ASH1, anticorps HASH1, anticorps MASH1, anticorps bHLHa46, anticorps AI225900, anticorps Mash1, anticorps ASCL1, anticorps Zash-1a, anticorps ash, anticorps asha, anticorps id:ibd5085, anticorps im:7138587, anticorps si:dkey-264k15.2, anticorps zash1a, anticorps zasha, anticorps Xash1, anticorps ascl1-A, anticorps Zash-1b, anticorps ashb, anticorps zashb, anticorps achaete-scute family bHLH transcription factor 1, anticorps achaete-scute family bHLH transcription factor 1a, anticorps achaete-scute family bHLH transcription factor 1 L homeolog, anticorps achaete-scute complex homolog 1, anticorps achaete-scute family bHLH transcription factor 1b, anticorps ASCL1, anticorps Ascl1, anticorps ascl1a, anticorps ascl1.L, anticorps LOC100220505, anticorps ascl1b

Sujet: MASH1 and MASH2 (Mammalian achaete-schute Homolog 1 and 2) are basic helixloop-helix transcription factors which are mammalian homologs of the achaete-schute gene family that is required for neuronal development in Drosophila. The bHLH motif is present in many types of transcription factors including E2-2, E47, MyoD and others. MASH1 has been shown to heterodimerize with other bHLH containing transcription factors. MASH1-E12 heteromers bind to and promote transcriptional activation of muscle creatine kinase, a known target for MyoD activation. However, MASH1 appears to play its primary role during early development of the autonomic nervous system. In committed neuronal precursor cells, early expression of MASH1 activates a subset of neuron-specific genes to promote differentiation. Thus null mutations in the MASH1 gene suggest that MASH1 is a valuable marker for investigation of neural development in mammals. MASH1 is observed as an ~34 kDa protein by SDS-PAGE.

Poids moléculaire: 34 kDa

Pathways: Dopaminergic Neurogenesis