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LY6C + LY6G anticorps

Reactivité: Souris FACS, WB, IHC (fro), IP, IHC (p), IHC (zinc), IHC (f), CyTox Hôte: Rat Monoclonal RB6-8C5 unconjugated
N° du produit ABIN967573
  • Antigène
    LY6C + LY6G
    Reactivité
    Souris
    Hôte
    • 50
    • 1
    Rat
    Clonalité
    • 50
    • 1
    Monoclonal
    Conjugué
    • 10
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Inconjugué
    Application
    • 44
    • 9
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Cytotoxicity Test (CyTox)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Not Reported
    Clone
    RB6-8C5
    Isotype
    IgG2b kappa
  • Indications d'application
    For immunohistochemistry, we recommend using purified RB6-8C5 antibody in our special formulation for IHC.
    Commentaires

    Related Products: ABIN967573

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4° C.
  • Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." dans: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).

    Rakhmilevich: "Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes." dans: Journal of leukocyte biology, Vol. 57, Issue 6, pp. 827-31, (1995) (PubMed).

    Conlan, North: "Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody." dans: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 259-68, (1994) (PubMed).

    Czuprynski, Brown, Maroushek, Wagner, Steinberg: "Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 4, pp. 1836-46, (1994) (PubMed).

    Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, (1993) (PubMed).

    Tepper, Coffman, Leder: "An eosinophil-dependent mechanism for the antitumor effect of interleukin-4." dans: Science (New York, N.Y.), Vol. 257, Issue 5069, pp. 548-51, (1992) (PubMed).

    Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).

    Jutila, Kroese, Jutila, Stall, Fiering, Herzenberg, Berg, Butcher: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." dans: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, (1989) (PubMed).

  • Antigène
    LY6C + LY6G
    Autre désignation
    Ly-6G and Ly-6C
    Sujet
    The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the lineage cocktail used in studies of hematopoietic lineages. The mAb 1A8 specifically recognizes Ly-6G, but not Ly-6C. This antibody is routinely tested by flow cytometric analysis.
    Synonyms: Gr-1
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