Btk and Itk (pTyr551) anticorps

N° du produit ABIN967576

Détail du produit

Antigène: Btk and Itk

Épitope: pTyr551

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Marque: BD Pharmingen™

Attributs du produit: 1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human Btk

Clone: 24a-BTK

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Référence

Wilcox, Berg: "Itk phosphorylation sites are required for functional activity in primary T cells." dans: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37112-21, (2003) (PubMed).

Rawlings, Scharenberg, Park, Wahl, Lin, Kato, Fluckiger, Witte, Kinet: "Activation of BTK by a phosphorylation mechanism initiated by SRC family kinases." dans: Science (New York, N.Y.), Vol. 271, Issue 5250, pp. 822-5, (1996) (PubMed).

Mahajan, Fargnoli, Burkhardt, Kut, Saouaf, Bolen: "Src family protein tyrosine kinases induce autoactivation of Bruton's tyrosine kinase." dans: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5304-11, (1995) (PubMed).

Détail du antigène

Antigène: Btk and Itk

Sujet: Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. The activity of Btk is regulated by Src mediated phosphorylation of the kinase domain at tyrosine 551 (Y551). This event induces Btk kinase activity and subsequent autophosphorylation at Y223. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The Tec family kinase Itk plays a critical role in signal transduction downstream of the T cell antigen receptor and has been implicated in the activation of phospholipase C-gamma1 (PLCgamma1), a key regulator of calcium mobilization and extracellular signal-regulated kinase (ERK) activation. Itk is regulated by an activating transphosphorylation event in which Y511 in the kinase domain is phosphorylated by Lck. The 24a/BTK (Y551) monoclonal antibody recognizes the Y551-phosphorylated form of human Btk and the Y511 phosphorylated form of human Itk.