Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

LAT anticorps (pTyr226)

LAT Reactivité: Humain WB, IHC (f) Hôte: Souris Monoclonal J96-1238-58-93 unconjugated
N° du produit ABIN967594
  • Antigène Voir toutes LAT Anticorps
    LAT (Linker For Activation of T Cells (LAT))
    Épitope
    • 19
    • 15
    • 15
    • 8
    • 8
    • 7
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr226
    Reactivité
    • 134
    • 59
    • 41
    • 1
    • 1
    Humain
    Hôte
    • 117
    • 17
    • 2
    • 1
    Souris
    Clonalité
    • 119
    • 18
    Monoclonal
    Conjugué
    • 65
    • 8
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    Cet anticorp LAT est non-conjugé
    Application
    • 74
    • 41
    • 39
    • 39
    • 18
    • 12
    • 11
    • 9
    • 6
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Phosphorylated Human LAT Peptide
    Clone
    J96-1238-58-93
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product LAT Anticorps primaire
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Janssen, Zhang: "Adaptor proteins in lymphocyte activation." dans: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).

    Samelson: "Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins." dans: Annual review of immunology, Vol. 20, pp. 371-94, (2002) (PubMed).

    Zhu, Janssen, Zhang: "Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 325-33, (2002) (PubMed).

    Lin, Weiss: "Identification of the minimal tyrosine residues required for linker for activation of T cell function." dans: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 29588-95, (2001) (PubMed).

    Paz, Wang, Clarke, Lu, Stokoe, Abo: "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells." dans: The Biochemical journal, Vol. 356, Issue Pt 2, pp. 461-71, (2001) (PubMed).

  • Antigène
    LAT (Linker For Activation of T Cells (LAT))
    Autre désignation
    LAT (LAT Produits)
    Synonymes
    anticorps LAT, anticorps lat, anticorps LAT1, anticorps pp36, anticorps linker for activation of T-cells, anticorps linker for activation of T cells, anticorps LAT, anticorps lat, anticorps Lat
    Sujet
    Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCgamma1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development.
    The J96-1238.58.93 monoclonal antibody recognizes the phosphorylated tyrosine 226 (pY226) of LAT, which is one of the phosphotyrosine sites required for binding Vav, Grb2, and Gads.
    Poids moléculaire
    38 kDa
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Vous êtes ici:
Support technique