Retinoblastoma 1 anticorps (pSer780)
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- Antigène Voir toutes Retinoblastoma 1 (RB1) Anticorps
- Retinoblastoma 1 (RB1)
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Épitope
- pSer780
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Retinoblastoma 1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Marque
- BD Pharmingen™
- Attributs du produit
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Phosphorylated Human Rb
- Clone
- J146-35
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product RB1 Anticorps primaire
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- Commentaires
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Related Products: ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C.
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Pocket proteins and cell cycle control." dans: Oncogene, Vol. 24, Issue 17, pp. 2796-809, (2005) (PubMed).
: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." dans: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, (1997) (PubMed).
: "
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Pocket proteins and cell cycle control." dans: Oncogene, Vol. 24, Issue 17, pp. 2796-809, (2005) (PubMed).
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- Antigène
- Retinoblastoma 1 (RB1)
- Autre désignation
- Rb (RB1 Produits)
- Synonymes
- anticorps OSRC, anticorps RB, anticorps p105-Rb, anticorps pRb, anticorps pp110, anticorps Rb, anticorps Rb-1, anticorps P105-RB, anticorps PP110, anticorps RB11, anticorps zgc:154147, anticorps RB transcriptional corepressor 1, anticorps RB transcriptional corepressor like 1, anticorps retinoblastoma 1, anticorps RB1, anticorps RBL1, anticorps Rb1, anticorps rb1
- Sujet
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The retinoblastoma gene product (Rb) is well known as a tumor suppressor and is either absent or mutated in many human tumors. Retrovirus-mediated gene transfer of the wild-type Rb gene into several Rb mutant neoplastic cell lines suppresses their tumorgenicity. Rb is a 110-kDa nuclear phosphoprotein that undergoes differential phosphorylation during the cell cycle. During G1 phase, Rb is predominantly in a hypophosphorylated state. It becomes increasingly phosphorylated throughout the cell cycle until late mitosis, when substantial dephosphorylation occurs. Hypophosphorylated Rb interacts with a number of cellular proteins including the E2F transcription factor, several cyclins, RBP-1, RBP-2, c-Abl, c-myc, N-myc, and p46. Phosphorylation of Rb at various sites, by Cyclin-dependent protein kinases, inhibits the binding of Rb to these proteins. Rb is thought to mediate its effects, in part, via the repression of genes required for proliferation. For example, Rb is specifically recruited to promoters containing E2F sites and actively represses E2F mediated transcription. Rb also stimulates the activity of other transcription factors, although the mechanisms are less clearly defined. Thus, Rb appears to regulate transcription in its aim to control cell growth.
The J146-35 monoclonal antibody recognizes Rb phosphorylated at serine 780 (pS780), which affects Rb binding to E2F. The orthologous phosphorylation sites in mouse and rat Rb are serines 773 and 751, respectively. - Poids moléculaire
- 110 kDa
- Pathways
- Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
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