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NFkB anticorps (pSer529)

NFkB Reactivité: Humain WB, ICS Hôte: Souris Monoclonal K10-895-12-50 unconjugated
N° du produit ABIN967610
  • Antigène Voir toutes NFkB Anticorps
    NFkB (Nuclear Factor kappa B (NFkB))
    Épitope
    • 10
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer529
    Reactivité
    • 27
    • 7
    • 3
    • 3
    • 1
    Humain
    Hôte
    • 31
    • 3
    Souris
    Clonalité
    • 30
    • 4
    Monoclonal
    Conjugué
    • 19
    • 6
    • 4
    • 2
    • 2
    • 2
    Cet anticorp NFkB est non-conjugé
    Application
    • 26
    • 16
    • 15
    • 8
    • 8
    • 7
    • 5
    • 2
    • 1
    Western Blotting (WB), Intracellular Staining (ICS)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Please refer to us for technical protocols.
    2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Phosphorylated Human NF-kappaB p65 Peptide
    Clone
    K10-895-12-50
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product NFkB Anticorps primaire
  • Commentaires

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Natoli, Saccani, Bosisio, Marazzi: "Interactions of NF-kappaB with chromatin: the art of being at the right place at the right time." dans: Nature immunology, Vol. 6, Issue 5, pp. 439-45, (2005) (PubMed).

    Siebenlist, Brown, Claudio: "Control of lymphocyte development by nuclear factor-kappaB." dans: Nature reviews. Immunology, Vol. 5, Issue 6, pp. 435-45, (2005) (PubMed).

    Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." dans: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, (2005) (PubMed).

  • Antigène
    NFkB (Nuclear Factor kappa B (NFkB))
    Autre désignation
    NF-kappa B (NFkB Produits)
    Synonymes
    anticorps EBP-1, anticorps KBF1, anticorps NF-kB1, anticorps NF-kappa-B, anticorps NF-kappaB, anticorps NFKB-p105, anticorps NFKB-p50, anticorps NFkappaB, anticorps p105, anticorps p50, anticorps NF-kB, anticorps NF-KB1, anticorps NF-kappaB1, anticorps p50/p105, anticorps nuclear factor kappa B subunit 1, anticorps nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105, anticorps NFKB1, anticorps Nfkb1
    Sujet
    Nuclear factor kB (NF-kB) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF-kB complex consists of the p50 (also known as NF-kB1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor IkB and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkB. This complex prevents nuclear translocation and activity of NF-kB. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, IkB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkB/NF-kB complex, allowing the free heterodimeric NF-kB to translocate to the nucleus. Furthermore, optimal activation of NF-kB requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF-kB dimers bind to the kB sites within promoters and enhancers and function as transcriptional activators. The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of human NF-kB p65 subunit.
    Poids moléculaire
    65 kDa
    Pathways
    Ubiquitin Proteasome Pathway, S100 Proteins
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