PDGFRA anticorps

N° du produit ABIN967626

Détail du produit anti-PDGFRA anticorps

Antigène: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp PDGFRA est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse PDGF Receptor alpha chain

Clone: APA5

Isotype: IgG2a kappa

Information d'application

Indications d'application: This antibody has been tested by immunofluorescent staining (Less or equal than 1 µg/million cells) with flow cytometric analysis and immunohistochemical staining of acetone-fixed frozen sections (0.5 - 2.5 µg/ml) to assure specificity and reactivity. Other reported applications include western blot analysis, ELISA, immunohistochemical staining of paraffin-embedded sections, inhibition of ligand binding, and in vivo blocking of PDGF action.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-PDGFRA (ABIN967626)

Fruttiger, Calver, Krüger, Mudhar, Michalovich, Takakura, Nishikawa, Richardson: "PDGF mediates a neuron-astrocyte interaction in the developing retina." dans: Neuron, Vol. 17, Issue 6, pp. 1117-31, (1997) (PubMed).

Takakura, Yoshida, Kunisada, Nishikawa, Nishikawa: "Involvement of platelet-derived growth factor receptor-alpha in hair canal formation." dans: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 770-7, (1996) (PubMed).

Heldin: "Structural and functional studies on platelet-derived growth factor." dans: The EMBO journal, Vol. 11, Issue 12, pp. 4251-9, (1992) (PubMed).

Détails sur PDGFRA

Antigène: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Autre désignation: CD140a (PDGFRA Produits)

Synonymes: anticorps AI115593, anticorps CD140a, anticorps Pdgfr-2, anticorps APDGFR, anticorps PDGFACE, anticorps CD140A, anticorps PDGFR-2, anticorps PDGFR2, anticorps RHEPDGFRA, anticorps PDGFR, anticorps PDGFR-A, anticorps platelet derived growth factor receptor alpha, anticorps platelet derived growth factor receptor, alpha polypeptide, anticorps platelet-derived growth factor receptor, alpha polypeptide L homeolog, anticorps platelet-derived growth factor receptor, alpha polypeptide, anticorps PDGFRA, anticorps Pdgfra, anticorps pdgfra.L

Sujet: The APA5 antibody reacts with Platelet-Derived Growth Factor (PDGF) Receptor alpha chain (CD140a, PDGFR-a), a receptor tyrosine kinase which is widely expressed on cells of mesenchymal origin in the embryo and adult and on several other cell types during embryonic development, but not on hematopoietic cells. PDGFR-a binds to PDGF A and B chains, in contrast to PDGFR-b, which binds only to the PDGF B chain. Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Binding to PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization. The mAb APA5 has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo.
Synonyms: PDGF Receptor alpha chain

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling